Biol 240 - UW - Final Exam Questions And Answers With Verified Solutions Graded A+

· Turbidity Measurements - Spectrophotometer sends light through a culture. Light absorbance can give a rough measure of cell density in the tube ·Agrobacterium - Crown-Gall Disease - The crown gall is where the stem of the plant connects with the roots of the plant. Causes tumours, parasitism. THIS IS BAD, we don't want this one. Agrobacterium is the only one that can use opine as a carbon and nitrogen source. It robs the plant of organic carbon. We can use agrobacterium to introduce new genes into plants 0157 - E Coli on lettuce is a shiga toxin and makes you very sick, it doesn't make cows sick. Cows put it into water and it gets on our lettuce and our intestines have receptors for it so that it can affect us 2D PAGE Analysis - Across the top of the gel you spread your proteins based on their pH. They move through the gel until they reach a point where they become neutral, isoelectric point. Small proteins move further down than the larger proteins 2D-Page - Allows separation of proteins on a gel based on isoelectric point (i.e. charge, pH where the protein has no charge). Mass spectrometry can determine amino acid sequences of the polypeptides from 2D-PAGE. Comparison of sequence to known protein sequences can help determine identity A broad spectrum antibiotic treatment as quickly as possible! - Ampicillin and Tetracycline A common property to all recognition sites... - Is that they all dictate where both strands need to be cut A human feels sick and asks about the pathogens cell wall. Which cell wall composition can you rule out? - NAG and NAT (these are archaea) A new halophile in the dead sea was found. What will its cell wall look like? - It is an archaea so something with NAG and NATA new organism that uses light for energy and grows on acetate and other organic acids. What is this organism classified as? - Photoorganoheterotroph A serial dilution of a culture was performed. The dilution factor is 10-3. After 1 ml of diluted solution was included in a pour plate and incubated, 40 colonies grew. How many colony forming units per ml were present in the original culture? - 40000 CFU per ml A single response regulator can be... - An activator, a repressor, or both A wild type is met+ pro-. Which defined medium should you use to recover a met+ pro+ mutant? - Medium without methionine nor proline