Biosafety Level Questions and Answers Already Passed

Biosafety Level Questions and Answers Already Passed **Question 1:** What is the definition of biohazardous materials? Answer: Biohazardous materials are agents of biological origin that have the potential to cause harm or adverse effects on humans, animals, plants, and the environment. **Question 2:** What are the different types of biohazardous materials? Answer: The list includes viruses, bacteria, fungi, recombinant DNA, toxins and allergens derived from microorganisms, allergens and toxins derived from higher plants and animals, transgenic plants, animals, and insects, human and primate cells, tissues, and body fluids, and viral vectors. **Question 3:** How many hazard groups do biological agents need to be classified into? Answer: Biological agents must be classified into 4 hazard groups based on their potential risk to human health. **Question 4:** What are the characteristics of each hazard group? Answer: Group 1: unlikely to cause human disease; Group 2: can cause human disease but is unlikely to spread to the community and has effective treatment and prevention measures; Group 3: causes severe human disease but has effective treatment and prevention measures; Group 4: causes severe human disease and has no effective treatment or prevention measures. **Question 5:** What is the minimum containment level required for each hazard group? Answer: Hazard Group 1 requires BSL 1, Hazard Group 2 requires BSL 2, Hazard Group 3 requires BSL 3, and Hazard Group 4 requires BSL 4. **Question 6:** What does containment refer to in the context of biohazardous materials? Answer: Containment refers to the safe methods for managing infectious materials in a laboratory environment where they are being handled or maintained. What are the "Good Microbiological Techniques (GMT)"? - using proper aseptic techniques; - wearing appropriate personal protective equipment (PPE); - minimizing the production of aerosols or working in a biological safety cabinet (BSC) when aerosol production is unavoidable; - employing universal precautions with blood borne pathogens; - routinely disinfecting work surfaces and equipment/supplies used in the experiment; - immediately cleaning spills according to laboratory protocols; - restricting work with infectious material to one