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MICR 4423 Exam 5 questions with correct answers

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What is antibiotic selection? Correct Answer-Survival of a population of antibiotic resistant cells within a larger population of antibiotic sensitive cells. Where can antibiotic selection occur? Correct Answer-Anywhere where there are antibiotics and bacteria present. The best-surviving bacteria will be selected for. Name a common use for antibiotic selection in microbiological research. Correct Answer-To identify and isolate cells that have piece of DNA of interest such as a particular plasmid or gene in the chromosome. Why does virtually every plasmid used for scientific research contain an antibiotic resistance marker? Correct Answer-It allows the cells that contain that plasmid to be identified through antibiotic selection. Imagine that you have a sample of bacteria that is a mixture of ampicillin-resistant cells and ampicillin-sensitive cells. How could you isolate the ampicillin-resistant cells? Correct Answer-Plate the cells on an ampicillin plate so that only the ampicillin-resistant cells will survive and produce colonies. What antibiotic or class of antibiotics do cells containing the bla marker become resistant to? What about aac? What about cat? Correct Answer-Bla- ampicillin/any cillin Aac- aminoglycosides (usually kanamycin) Cat- chloramphenicol

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