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Microbiology UTA 2460 - Lab Practical 2 Correctly Answered Questions| UpToDate | Already Graded A+

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What is the role of resazurin in fluid thioglycolate medium... : Resazurin indicates the presence of oxygen in the media A bacterial isolate is subcultured to two blood agar plates, one of which is incubated in an anaerobe jar, and the other is incubated on a shelf in the incubator. After incubation, growth is observed on the plate from the anaerobe jar, but there is not growth on the plate incubated on the incubator shelf. Based on these results how would this organism be categorized... : obligate anaerobe Where would you predict an facultative anaerobe would grow in a tube of fluid thioglycolate medium... : throughout the tube The pink color in the top of the broth in an FTM tube indicates... : the presence of oxygen Both human pathogens and normal microbiota are typically classified as... : mesophiles 2 | P a g e Taq polymersae is a DNA polymerase enzyme that demonstrates maximal activity at 72C. The organism from which this enzyme was isolated would most likely be classified as a... : thermophile Vibrio cholerae is the bacterium responsible for cholera, a GI tract infection. Normally, it takes the ingestion of over one million bacterial cells to cause infection, but the consumption of antacids significantly lowers this number. What does this tell you about the likely classification of V. cholerae in terms of pH preference... : it is likely an alkaliphile How does pH negatively affect the metabolism of microorganisms... : pH changes can denature enzymes Why can jams and honey be stored at room temperature for long periods of time... : the high sugar content causes plasmolysis of bacterial cells Microorganisms that can grow at salt concentrations as high as 15-30% are known as... : halophiles An experiment involves the inoculation bacteria onto a variety of media with NaCl concentrations ranging from 1% to 25%. Which of these media is most likely to be isotonic with the bacterial cells..

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Microbiology UTA 2460 - Lab Practical 2
Correctly Answered Questions | UpToDate |
Already Graded A+

What is the role of resazurin in fluid thioglycolate medium...

🗸🗸🗸: Resazurin indicates the presence of oxygen in the media



A bacterial isolate is subcultured to two blood agar plates, one of which is incubated in an

anaerobe jar, and the other is incubated on a shelf in the incubator. After incubation, growth is

observed on the plate from the anaerobe jar, but there is not growth on the plate incubated on the

incubator shelf. Based on these results how would this organism be categorized...

🗸🗸🗸: obligate anaerobe



Where would you predict an facultative anaerobe would grow in a tube of fluid thioglycolate

medium...

🗸🗸🗸: throughout the tube



The pink color in the top of the broth in an FTM tube indicates...

🗸🗸🗸: the presence of oxygen



Both human pathogens and normal microbiota are typically classified as...

🗸🗸🗸: mesophiles

1|Page

, Taq polymersae is a DNA polymerase enzyme that demonstrates maximal activity at 72C. The

organism from which this enzyme was isolated would most likely be classified as a...

🗸🗸🗸: thermophile



Vibrio cholerae is the bacterium responsible for cholera, a GI tract infection. Normally, it takes

the ingestion of over one million bacterial cells to cause infection, but the consumption of

antacids significantly lowers this number. What does this tell you about the likely classification

of V. cholerae in terms of pH preference...

🗸🗸🗸: it is likely an alkaliphile



How does pH negatively affect the metabolism of microorganisms...

🗸🗸🗸: pH changes can denature enzymes



Why can jams and honey be stored at room temperature for long periods of time...

🗸🗸🗸: the high sugar content causes plasmolysis of bacterial cells



Microorganisms that can grow at salt concentrations as high as 15-30% are known as...

🗸🗸🗸: halophiles



An experiment involves the inoculation bacteria onto a variety of media with NaCl

concentrations ranging from 1% to 25%. Which of these media is most likely to be isotonic with

the bacterial cells...

2|Page

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