Mmbio 221 Final Exam Study Guide 2024
MMBIO 221 FINAL EXAM STUDY GUIDE 2024 Who is the father of microscopy? Antonie van Leeuwenhoek Lamp: produces visible light to illuminate the specimen Condenser: collects light and directs it through the stage opening (converges or focuses the rays of light to a single point) Iris diaphragm: regulates the amount of light available to the objective lens Factors that affect microscope function: Resolving power, numerical aperture, magnification, working distance Resolving power: ability of a microscope to differentiate two closely spaced objects as being distinct from one another, use of shorter wavelength of light increases resolving power, resolving power also depends on a second factor: the numerical aperture Resolving power _____ as magnification of the objective lens ____ increases, increases Numerical aperture: ability of a lens in a light microscope to gather light Refraction: light rays are bent or refracted as they travel through media like glass and air, amount of bending is indicated by the refractive index. Immersion oil minimizes refraction producing a clearer image with more detail. Magnification: product of the magnification of the ocular (10x) and objective lens Working distance:
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