TLC Lab Report 5 Study Guide Rated A

TLC Lab Report 5 Study Guide Rated A+ Thin Layer Chromatography consists of three parts: The analyte, the stationary phase, and mobile phase. Match each of these terms to what it was in our experiment. 1. Mobile Phase 2. Analyte 3. Stationary Phase The dominate reason by which analytes are retained on a TLC plate is? If the distance from the origin (start line Figure 10.2) to the middle of a spot is 4.8 cm and the distance from the middle of the same spot to the solvent front (end line Figure 10.2) is 3.4 cm, what is the Rf for this compound? Round to 2 decimal places without units. Complete the sentences. 1. The least polar compound should have a(n) 2. The most polar compound should have a(n) Based on our TLC experiments, which analgesic is least polar? The least polar analgesic has... Based on our experiments, which analgesic is most polar? Complete the sentences. 1. Analytes on a TLC should have a(n) ______ Rf in a less polar solvent. 2. Analytes on a TLC should have a(n) ______ Rf in a more polar solvent. Based on our experiments, which solvent mixture was most polar? Based on our experiments, which solvent mixture was best for separating ibuprofen and naproxen? Hexanes is ... Match each concentration of acetic acid to its name. 1. Concentrated acetic acid that forms "icebergs" when cooled to its melting point below room temperature. 2. Dilute aqueous solutions of acetic acid. Of the solvents that we used in our TLC experiment, which is least polar? Hint: When a non-polar and polar solvent are mixed, the polar solvent dominates in the overall polarity of the solvent. The compounds that penetrate the human body best are ... The purpose of TLC is to... If you take a square TLC plate and develop a mixture with one solvent mixture, dry the plate, and develop it with another solvent mixture you can separate one set of compounds well and then separate another set of compounds well. In which directions should you develop the TLC plate with the two different solvent mixtures? This technique is often used to separate molecules of biological interest. Chromatography (color writing) was originally developed using paper to separate what?