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BIOD171 Microbiology Lab Notebook 2 2 1 5
Psychiatric/Mental Health Nursing (Santa Fe College)
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Portage Learning
BIO 171- Microbiology
Lab Notebook
Lab Notebook Bookmarks (click to navigate):
Lab 1 Notebook
Lab 2 Notebook
Lab 3 Notebook
Lab 4 Notebook
Lab 5 Notebook
Lab 6 Notebook
Lab 7 Notebook
Lab 8 Notebook
Lab 9 Notebook
1
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Lab 1 Notebook
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Title: KD01 Introduction to medical microbiology & lab notebook procedures
Course Objectives:
Cultivation of samples: growth conditions and equipment used
Identification of samples: biochemical assays & tests available
Evaluation of samples: microscopy- visualization and key characteristics of specimens
Basic Equipment:
Cleaning: Autoclave 125°C- uses heat, pressure, & steam to sterilize; autoclave will take
minutes to sterilize & hot dry air which will take hours; before
opening make sure it is depressurized and will have very hot air.
Growing (bacteria/pathogens) – Fixed incubator 37°C is airtight, shelving for petri dishes
- Shaker incubator 37°C shakes culture & rotates to aerate-
liquid nutrient broth
Visualizing: microscopy
Storing: refrigerator @ 4°C to stop the growth and helps preserve samples & keeps long term
Lab Safety
1. Never eat or drink in the lab: contamination risks
2. Use PPE (personal protection equipment): gloves- latex, nitrile (purple, blue,
green),thermal,
or cold gloves (liquid nitrogen): eyewear: lab coat- chemical spills
2
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3. Never leave lab while wearing PPE: including bathroom, hallway, or cafeteria
Outline of how to set up Notebook
Objective: To establish an organized template for keeping experimental records, procedures,
and results.
Procedure:
1. This is where each step of a given protocol is recorded
2. Be sure to clearly indicate any deviations that may occur during the experiment- put
them in red
Notes: Additional (helpful) information placed here
Results: A summary of the final outcome of the experiment should be described here
3
Downloaded by charity nimo ()
BIOD171 Microbiology Lab Notebook 2 2 1 5
Psychiatric/Mental Health Nursing (Santa Fe College)
Studocu is not sponsored or endorsed by any college or university
Downloaded by charity nimo ()
, lOMoAR cPSD| 22830792
20
Portage Learning
BIO 171- Microbiology
Lab Notebook
Lab Notebook Bookmarks (click to navigate):
Lab 1 Notebook
Lab 2 Notebook
Lab 3 Notebook
Lab 4 Notebook
Lab 5 Notebook
Lab 6 Notebook
Lab 7 Notebook
Lab 8 Notebook
Lab 9 Notebook
1
Downloaded by charity nimo ()
, lOMoAR cPSD| 22830792
Lab 1 Notebook
Back to Home Page
Title: KD01 Introduction to medical microbiology & lab notebook procedures
Course Objectives:
Cultivation of samples: growth conditions and equipment used
Identification of samples: biochemical assays & tests available
Evaluation of samples: microscopy- visualization and key characteristics of specimens
Basic Equipment:
Cleaning: Autoclave 125°C- uses heat, pressure, & steam to sterilize; autoclave will take
minutes to sterilize & hot dry air which will take hours; before
opening make sure it is depressurized and will have very hot air.
Growing (bacteria/pathogens) – Fixed incubator 37°C is airtight, shelving for petri dishes
- Shaker incubator 37°C shakes culture & rotates to aerate-
liquid nutrient broth
Visualizing: microscopy
Storing: refrigerator @ 4°C to stop the growth and helps preserve samples & keeps long term
Lab Safety
1. Never eat or drink in the lab: contamination risks
2. Use PPE (personal protection equipment): gloves- latex, nitrile (purple, blue,
green),thermal,
or cold gloves (liquid nitrogen): eyewear: lab coat- chemical spills
2
Downloaded by charity nimo ()
, lOMoAR cPSD| 22830792
3. Never leave lab while wearing PPE: including bathroom, hallway, or cafeteria
Outline of how to set up Notebook
Objective: To establish an organized template for keeping experimental records, procedures,
and results.
Procedure:
1. This is where each step of a given protocol is recorded
2. Be sure to clearly indicate any deviations that may occur during the experiment- put
them in red
Notes: Additional (helpful) information placed here
Results: A summary of the final outcome of the experiment should be described here
3
Downloaded by charity nimo ()
