QBM Final Exam Questions And Answers With Complete Solutions 2024

QBM Final Exam Questions And Answers With Complete Solutions 2024 What is transcription? - answerDNA RNA What is the best representation/model of proteins? - answerMolecular Surface Model Describe the four levels of protein structure. - answerPrimary: amino acid sequence Secondary: the primary chain forms spirals(alpha helix) and sheets(beta sheets) Tertiary: 3-D with folded motifs Quaternary structure: Two or more polypeptide chains, with its own tertiary structure, combine to form a functional protein. What does OSHA stand for? - answerOccupational Safety and Health Administration What is the role of OSHA? - answeremployers must inform employees of potential hazards What is included in the MSDS? - answerChemical's physical and chemical properties, toxic, carcinogenic etc. and describe the proper way of handling these chemicals Describe the four biosafety levels - answerBSL-1: Minimal hazards. BSL-2: Organisms with potential hazards; proper protective equipment; waste disposed properly; most teaching labs and some research labs. (EX:QBM lab) BSL-3: Pathogenic organisms that cause serious disease; proper ventilation; lab restriction; special protective equipment; some research labs BSL-4: Dangerous pathogens that cause severe to fatal disease; no vaccines or treatments List the from best to worst glove type - answerLatex Nitrile Plastic None how much should be used when using graduated cylinder? - answer25ml-2L What is a transfer/pasteur pipette used for? - answerquick measurements, non-accurate What is a micropipette used for? - answer0.1 ul to 1 ml accuracy, the tip in the 1 cm of liquid and held vertically. Describe an autoclave - answerDevice with high temperatures and pressure used to sterilize glassware and peptides Describe centrifugation - answerused to separate samples based on mass, shape and density Describe a fixed angle rotor? - answermost common, particles pellet to a side when centrifuged Describe a swinging bucket rotor? - answerdoes NOT creat tight pellets, for sample analysis or running cesium chloride or sucrose density gradients. Used to separate by density. Describe clear (polycarbonate) bottles - answerBetter visibility, less resistant to chemicals, can weaken/crack Describe opaque (polypropylene) bottles - answerMore resistant to chemicals and cracks Describe Spectroscopy - answerstudy of radiation interacting with matter, can analyze molecular structure (can be