QBM Final Exam Questions With 100% Correct Answers

QBM Final Exam Questions With 100% Correct Answers What is the definition of Molecular biology - answerStructure and function of macromolecules (proteins and nucleic acids) What is the central dogma of DNA? - answerDNA ⟶ RNA ⟶ Proteins What BSL corresponds to minimal hazards? - answerBSL-1 What does BSL-1 correspond to? - answerminimal hazards What BSL corresponds to organisms with potential hazards? - answerBSL-2 What BSL(s) require proper protective equipment? - answerBSL2-4 Under what BSL do most teaching labs and some research labs fall under? - answerBSL-2 What BSL corresponds to labs that work with pathogenic organisms that cause serious disease? - answerBSL-3 What BSL corresponds to labs that work with dangerous pathogens that cause severe to fatal disease without vaccines or treatments? - answerBSL-4 What does MSDS stand for? - answermaterial safety data sheet What does the MSDS tell you about a chemical? - answerits physical, and chemical properties, its physical and health hazards, its primary route of entry, and its toxicity What are the OSHA requirements? - answerwritten hazard communication What does PPE stand for? - answerpersonal protective equipment Rank glove materials in order of decreasing goodness - answernitrile > latex > plastic > none What would you use to transfer something between 25 ml to 2 L? - answergraduated cylinder What would you use to transfer something from 1ml to 25 ml? - answerMohr and serological pipettes If you want a quick, non-accurate pipette what can you use? - answerTransfer/pasteur pipette If you want to transfer something with 0.1 microlitres to 1 mL what would you use? - answermicropipette What does analytical centrifugation study? - answersedimentation, determiens sample purity, molecular weight At what RPM does analytical centrifugation occur? - answer13000 rpm What is the most common type of centrifugation? - answerpreparative What is high speed centrifugation used for? - answerlarge volumes (25,000 rpm) What does a sucrose gradient centrifugation allow you to do? - answerseparate samples based on density What are the most common types of rotors? - answerfixed angle What are fixed angle rotors good for? - answerpelleting What are swinging bucket rotors not good at? - answercreating tight pellets what are swinging bucket rotors used for? - answersample analysis or running caesium chloride or sucrose density gradients What are the advantages of clear bottles? - answerbetter visibility What are the disadvantages of clear bottles? - answerless resistant to chemicals What is the advantage of opaque bottles? - answermore resistant to cehmicals What is spectroscopy? - answerThe study of radiation interacting with matter Is infrared spectroscopy precise? - answerno What is fluorescence used to determine? - answerstudies biological system What does NMR do? - answermeasures the spin state of proton by magnetic feild What does X-Ray spectroscopy do? - answerstudies the structures of cells, proteins and DNA What does CD spectroscopy measure? - answerdifferences in the absorption of left-handed polarised light vs right handed polarised light. Allows to get an idea of the secondary structure of the protein Spectrometry - answerMeasurements of the spectroscopy interactions, allowing you to determine the molecular structures What does the spectrophotometer measure? - answerthe intensity of a specific wavelength as it passes through a sample and compares it to a blank What are the five components of a spectrophotometer? - answerlight source, monochromator, prism, sample compartment, detector Plate readers - answerQuickly read many samples from a single plate What is measured at 260 nm - answerDNA abosrbance What is measured at 280 nm - answerProtein absorbance What is measured at 340 nm? - answerCDNB assay What is measured at 560 nm? - answerBCA assay What is measured at 595 nm? - answerBradford assay What is measured at 600 nm? - answerbacterial growth in LB media What is measured at 750 nm? - answerLowry/DC protein assay As transmission goes up what happens to concentration? - answerconcentration goes down What does an A 260 of 1 mean in terms of DNA concentration? - answer50 micrograms/millilter What is considered a pure dna sample? - answer260/280 > 1.5 What is the most accurate form of determine protein concentration? - answeramino acid analysis What are the three colorimetric methods of determining protein concentration? - answerbradford, bruit, lowryDC What does the DC assay allow ? - answerdetergents to be used What assay is the DC assay a modified form of? - answerLowry What assay uses coomassie blue? - answerbradford What colorimetric method of protein concentration is fastest? - answerbradford What does a standard curve relate? - answerabsorbance (y-axis) to concentration (x-axis) What assays use a standard curve? - answerBSA, Bradford, Lowry What do enzymes do? - answerspeed up the reaction by lowering activation energy