Histotechnology Exam Questions with Guaranteed Accurate Answers
What method is used to stabilize proteins making it resistant to further damage? - correct answer Fixation Name two methods of fixation? - correct answer Chemical reaction (formaldehyde) and physical (heat or dessication) What are two causes of Post mortem putrefaction if the tissue is not fixed? ***** - correct answer bacteria or mold What factor causes autolysis if tissue is not fixed? **** - correct answer enzyme attack What are four functions of fixatives? - correct answer 1. kills tissue to prevent putrefaction and autolysis 2. maintains proper relationship between intracellular and extracellular substances 3. preserves refractive indexes 4. enhances stains What three organs contain the highest levels of enzymes? - correct answer liver, kidney, and brain True or False. You can over fix tissue? - correct answer True. Over fixation dries out the tissue and limits the quality. Can attempt steaming the sections to remedy over fixation. If you receive a breast sample on a Friday night, what method must be performed to prevent exceeding the allowed formalin fixation time to perform the Her2 test? - correct answer Must place the tissue in 70%
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