Microbiology Lab 1 Practical Exam Questions and Answers

Microbiology Lab 1 Practical Exam Questions and Answers Magnification -Answer-makes an image appear larger then its actual size Total magnification -Answer-eyepiece x objective wavelength of light -Answer-Light travels as waves and wavelength is the distance peak to peak on a wave Visible light has a wavelength between 350-700 nanometers Each wavelength is a different color of light Violet = 350 nm, Red = 700nm For good resolution, we use blue light (480/500 nm) Numerical Aperture -Answer-Ability of a lens to gather light Working Distance -Answer-space between the stage and the objective lens when the specimen is in focus Par focal -Answer-once the specimen is in focus, you can change the objective lens and the specimen remains in course focus Field of View -Answer-circle of light observed through microscope As magnification increases, field of view decreases Ocular Lens -Answer-10x Scanning Objective Lens -Answer-4x Lower power objective -Answer-10x high power objective lens -Answer-40x oil immersion lens -Answer-100x Cocci -Answer-spherical Bacillus -Answer-Rod Shape spirilla -Answer-spiral shape Eubacteria -Answer-true bacteria Cyanbacteria -Answer-make their own food by photosynthesis, they are bacteria but not conform to any three shapes. - consist of prokaryotic cell Genus: Anabaena -Answer-Name Genus and Phylum Genus: Oscillatoria -Answer-Name Genus and Phylum Genus: Spirulina -Answer-Name Genus and Phylum Specie: Bacillus Subtilis -Answer-Name genus, specie, and shape Specie: Proteus vulgaris -Answer-Name Species and genus Specie: Staphylococcus aureus Genus: Bacteria Shape: cocci -Answer-Name genus, specie, and shape Streptococcus pyogenes Genus: bacteria Shape: cocci -Answer-Name genus, specie, and shape Species: Spirillum sp. Genus: bacteria Shape: Spirilla -Answer-Name genus, specie, and shape Saccharomyces cerevisiae