Carsons Histotechnology with 100% c0rrect answers 2024
Carsons Mordant . substance’s or metals that act as a link between dye and tissue A mordant is a metal with a valency of at least two. The two commonest metals used in histotechnology are aluminum and ferric iron, both with valencies of three. The attachment of mordant to dyes is by means of a covalent and a coordinate bond. Fixative additive fixation- stabilizes proteins by denaturing proteins (chemical, heat) and combines with fixative molecule becoming insoluble non additive fixative (alcohol, acetone)-denatured proteins dissociate bound water molecules but molecules do not combine prevents decay, purification and autolysis enhances staining w exception of masking antigenic sites but retrievable using antigen retrieval techniques makes tissue firmer for grossing protein stabilizing of fixatives renders enzymes inactive as result of protein stabilizing action/denaturing tissues rich in enzymes more susceptible to autolysis ie brain, pancreas, liver
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