Histopathologic Techniques: Fixation, Chapter 3 Part I (Gregorios) 2024 passed
Histopathologic Techniques: Fixation, Chapter 3 Part I (Gregorios)Fixation - correct answers the first and most critical step in histotechnology involves fixing and preserving fresh tissue for examination first aim: preserve cell (life-like) second aim: harden and protect tissues most important: stabilization of proteins Additive fixation and Non-additive fixation - correct answers the two basic mechanisms involved in fixation Additive fixation - correct answers the chemical constituents of the fixative is taken in and becomes part of the tissue by forming cross-links or molecular complexes and giving stability to the protein e.g., formalin, mercury, and osmium tetroxide Non-additive fixation - correct answers fixing agent is not incorporated into the tissue, but alters the tissue composition and stabilizes the tissue by removing the bound water attached to H-bonds of certain groups within the protein molecule e.g., alcohol fixatives pH 6 and 8 - correct answers satisfactory hydrogen ion concentration pH Room temperature - correct answers for surgical specimens 0-4°C - correct answers for electron microscopy and some histochemistry ideal temperature
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