bld 313 exam3 quizzes 56 Questions with 100% Correct Answers | Verified | Latest Update
Which of the following is an example of semi-quantitative test result? - ️️3+ Which of the following signals is light based? - ️️scatter Which of the following under-estimates the concentration but not over-estimates? - ️️downward shift What is the purpose of the reagent blank in spectrophotometric analysis? - ️️to subtract the color contribution of the reagent Which of the following describes a specimen blank? - ️️subtract the light absorption of patient serum. Which of the following statement is true about lower limit of detection and reagent blank? - ️️The distance between the lowest limit of detection and a reagent blank need to be at least 2SD+2SD. What is the molar absorptivity (ε) of a compound which produces an absorbance (A) of 0.250 at a concentration of 0.200 M in a cuvette path width of 2 cm? - ️️0.625 /(M• cm) A chromophore has a molecular weight of 150 g/mol. When 20.0 mg of this substance is placed in 500 mL of water, it produced an absorbance of 0.250. What is the molar absorptivity (ε) of this compound? (The cuvette path width is 1 cm). - ️️938 /(M• cm) The concentration of a solution is 10.0 M. It has an absorbance of 0.200 when placed in a 1.00 cm cuvette at the wavelength of 250 nm. What would be the absorbance if the solution is 5.00 M? Assume both concentrations are in the analytical range (within linear) - ️️0.100
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