TSIA and KIA test results and interpretations questions & answers 2024

TSIA and KIA test results and interpretations questions & answers 2024
yellow slant/yellow butt-KIA - ANSWER-glucose and lactose fermentation with acid accumulation in slant and butt.
symbol A/A
yellow slant/yellow butt-TSIA - ANSWER-glucose fermentation with acid production; proteins catabolized
aerobically (in the slant) with alkaline products symbol A/A
red slant/yellow butt - ANSWER-no fermentation;peptone catabolized aerobically and anaerobically with alkaline products; isolate is not from Enterobacteriaceae symbol K/K
red slant/no change in butt - ANSWER-no fermentation; peptone catabolized aerobically and anaerobically with alkaline products (reversion)
symbol K/NC
no change in slant/no change in butt - ANSWER-organism is growing slowly or not at all; isolate is not from Enterobacteriase symbol NC/NC
black precipitate in the agar - ANSWER-sulfur reduction (an acid condition from glucose fermentation of glucose, exists *********** even if the yellow color is obscured by the black precipitate)
symbol H2S
cracks in or lifting the agar - ANSWER-gas production