Microbiology Lab Exam 1 Note Cards
2023/ 53 Questions and Answers/ 100%
Correct.
when viewing a smear with the oil immersion lens the final magnification is - -
1000x;10x ocular x 100X objective
-what is the purpose of heat fixing - -kill the bacteria, adhere to glass anddenature the
proteins of bacteria
-name oneof the stains or regents used in the gram stain procedure - -gram crystal
violet, gram iodine, gram sfaranin, 95% ethanol
-why are aseptic techniques used in the lab? - -to ensure that we do not contaminate
what we are working with as as to ensue that we do not acciently inoculate ouselves.
example of aseptic techniques are flaming the loop/needle sterilizing it, breifly flaming
the test tube before and after obtaining the sample.
-the gram reaction is due to differences in the structure of wat part of the microbe - -its
cell wall, specifically peptidoglycan, gram positive bacteria have thicker peptidoglycan
layer compared to GN bacteria.
-Name 2 inoculating tools you will be using in this lab - -loop, needle
-what color is a gram positive microbe - -purple
-waht is the function of the ethanol in the gram stain procedure - -decolorize
-name one of the strains in the acid fast stain - -cold kinyoun, carbol fuschsin,
methylen blue
, -name one of the stains in the endospore stain - -malachite green, safranin
-what is the purpose of the acid-fast stain - -the purpose of the acid-fast stain is to
differentiate between the myobactriace family of bacteria from all other bacteria
-what color does na acid-fast microbe stain with acd-fast stain - -red
-what is the function of agarin the media - -agar allows media to remain solid at room
temperature
-what is the difference between broth and agar based media - -broth is liquid media at
room temperature, agar media is solid at room temp.
-what is the funciton of media - -media is used to provide nutrients to a microbe as well
as a growth medium
-what is the name of the plate inoculation technique - -quadrant steak plate
-Why must the media we are making today be sterilized prior to us? - -the meda must
be sterilized to remove any bacterial contaminant that may be introduced when the
media is made
-how do you know yo have dont a successful streak plate? - -youve ended up with
isolated colonies
-what do you think will happen if you inoculated an obligate anerobe onto a TSA plate
and incubated the incubator we have been using? - -the obligate anerobe would not
grow because it doesn't have the ability to produce th necessary enzymes to dtoxify toxic
oxygen species
-What is one procedure used to determine oxygen requirement of a micrboe - -
thioglycollate medium or anaerobic bag
2023/ 53 Questions and Answers/ 100%
Correct.
when viewing a smear with the oil immersion lens the final magnification is - -
1000x;10x ocular x 100X objective
-what is the purpose of heat fixing - -kill the bacteria, adhere to glass anddenature the
proteins of bacteria
-name oneof the stains or regents used in the gram stain procedure - -gram crystal
violet, gram iodine, gram sfaranin, 95% ethanol
-why are aseptic techniques used in the lab? - -to ensure that we do not contaminate
what we are working with as as to ensue that we do not acciently inoculate ouselves.
example of aseptic techniques are flaming the loop/needle sterilizing it, breifly flaming
the test tube before and after obtaining the sample.
-the gram reaction is due to differences in the structure of wat part of the microbe - -its
cell wall, specifically peptidoglycan, gram positive bacteria have thicker peptidoglycan
layer compared to GN bacteria.
-Name 2 inoculating tools you will be using in this lab - -loop, needle
-what color is a gram positive microbe - -purple
-waht is the function of the ethanol in the gram stain procedure - -decolorize
-name one of the strains in the acid fast stain - -cold kinyoun, carbol fuschsin,
methylen blue
, -name one of the stains in the endospore stain - -malachite green, safranin
-what is the purpose of the acid-fast stain - -the purpose of the acid-fast stain is to
differentiate between the myobactriace family of bacteria from all other bacteria
-what color does na acid-fast microbe stain with acd-fast stain - -red
-what is the function of agarin the media - -agar allows media to remain solid at room
temperature
-what is the difference between broth and agar based media - -broth is liquid media at
room temperature, agar media is solid at room temp.
-what is the funciton of media - -media is used to provide nutrients to a microbe as well
as a growth medium
-what is the name of the plate inoculation technique - -quadrant steak plate
-Why must the media we are making today be sterilized prior to us? - -the meda must
be sterilized to remove any bacterial contaminant that may be introduced when the
media is made
-how do you know yo have dont a successful streak plate? - -youve ended up with
isolated colonies
-what do you think will happen if you inoculated an obligate anerobe onto a TSA plate
and incubated the incubator we have been using? - -the obligate anerobe would not
grow because it doesn't have the ability to produce th necessary enzymes to dtoxify toxic
oxygen species
-What is one procedure used to determine oxygen requirement of a micrboe - -
thioglycollate medium or anaerobic bag
