Genetics Exam 4 (Final)
Study online at
https://quizlet.com/_cj9cgj
1. Disrupt target gene by
Genetics final exam prep, insert- ing neo+ and link tk+
to disrupted gene
(Answered) With Complete 2. transfer disrupted gene into
Verified Solutions
1. Place the steps of generating a gene -
knockout mouse in order from the dis-
ruption of the target gene to generat-
ing homozygous knockouts. Suppose
in
one experiment, the stem cells used are stem cells from a black mouse
from a black coated mouse, and the earl3y. allow recombination with
mouse embryo used is a white coated plas- mid that contains the
embryo. lacZ gene, an ori- gin of
replication, and an ampicillin
re- sistant gene. He inserts
the human in- sulin gene int
a restriction site locat- ed
within the lacZ gene. Then h
trans- forms this plasmid int
bacteria with- out a function
lacZ gene and spreads the
appropriate dilution onto
bacterial plates.
2. A geneticist isolates a new restriction
enzyme from the bacterium
Aeromonas ranidae. No other
restriction enzymes have been isolated
from this bacterial species.
Use the standard convention for abbre-
viating restriction enzymes to name
this new restriction enzyme.
3. Suppose a scientist wants to clone the
gene for human insulin. He uses a
, Genetics Exam 4 (Final)
Study online at
https://quizlet.com/_cj9cgj
genome to generate neo+ tk-
cells
4. select for neo+ and select
against tk+ to isolate cells from
homologous recombination
5.inject neo+ cells into white ear- ly
embryo and implant into surro- gate
6. identify chimeric progeny with
target-gene knockout cells
7. breed mice to generate ho-
mozygous knockouts
AraI
an LB agar plate with ampicillin and
X-Gal that produces color- less
colonies
, Genetics Exam 4 (Final)
Study online at
https://quizlet.com/_cj9cgj
When the product of the lacZ gene, be-
ta galactosidase, interacts with the
sub- trate X Gal on LB agar, colonies
turn blue.
Which plate indicates that the human
insulin gene was successfully inserted
in the plasmid?
4. Plasmids are small circular DNA mol- DNA from a gene of interest
ecules found in bacteria that can be inserted into a
replicate separately from plasmid, then the modified
chromosomes. plasmid can be in- serted
Why are plasmids essential for into a bacterial cell to repli-
recombi- nant DNA technology? cate a gene of interest many
times
5. Polymerase chain reaction (PCR) is a 2
technique used to amplify (copy)
DNA. Suppose a single, linear
molecule of
double stranded DNA (dsDNA) is ampli-
fied by PCR.
After one PCR cycle, how many
mole- cules of dsDNA will there be?
molecules of dsDNA after one cycle:
6. After three PCR cycles, how many mol- 8
ecules of dsDNA will there be?
7. After 30 PCR cycles (a typical number of ~1 billion
cycles), how many molecules of
dsDNA will there be?
8. Consider that a typical PCR does not 9. Suppose a scientist is
start with a single molecule of template studying the platypus,
DNA, but rather something in the range one of the few venomous
of 25 nmol of template DNA.
What does this tell you about the
poten- tial of PCR to amplify DNA?
Study online at
https://quizlet.com/_cj9cgj
1. Disrupt target gene by
Genetics final exam prep, insert- ing neo+ and link tk+
to disrupted gene
(Answered) With Complete 2. transfer disrupted gene into
Verified Solutions
1. Place the steps of generating a gene -
knockout mouse in order from the dis-
ruption of the target gene to generat-
ing homozygous knockouts. Suppose
in
one experiment, the stem cells used are stem cells from a black mouse
from a black coated mouse, and the earl3y. allow recombination with
mouse embryo used is a white coated plas- mid that contains the
embryo. lacZ gene, an ori- gin of
replication, and an ampicillin
re- sistant gene. He inserts
the human in- sulin gene int
a restriction site locat- ed
within the lacZ gene. Then h
trans- forms this plasmid int
bacteria with- out a function
lacZ gene and spreads the
appropriate dilution onto
bacterial plates.
2. A geneticist isolates a new restriction
enzyme from the bacterium
Aeromonas ranidae. No other
restriction enzymes have been isolated
from this bacterial species.
Use the standard convention for abbre-
viating restriction enzymes to name
this new restriction enzyme.
3. Suppose a scientist wants to clone the
gene for human insulin. He uses a
, Genetics Exam 4 (Final)
Study online at
https://quizlet.com/_cj9cgj
genome to generate neo+ tk-
cells
4. select for neo+ and select
against tk+ to isolate cells from
homologous recombination
5.inject neo+ cells into white ear- ly
embryo and implant into surro- gate
6. identify chimeric progeny with
target-gene knockout cells
7. breed mice to generate ho-
mozygous knockouts
AraI
an LB agar plate with ampicillin and
X-Gal that produces color- less
colonies
, Genetics Exam 4 (Final)
Study online at
https://quizlet.com/_cj9cgj
When the product of the lacZ gene, be-
ta galactosidase, interacts with the
sub- trate X Gal on LB agar, colonies
turn blue.
Which plate indicates that the human
insulin gene was successfully inserted
in the plasmid?
4. Plasmids are small circular DNA mol- DNA from a gene of interest
ecules found in bacteria that can be inserted into a
replicate separately from plasmid, then the modified
chromosomes. plasmid can be in- serted
Why are plasmids essential for into a bacterial cell to repli-
recombi- nant DNA technology? cate a gene of interest many
times
5. Polymerase chain reaction (PCR) is a 2
technique used to amplify (copy)
DNA. Suppose a single, linear
molecule of
double stranded DNA (dsDNA) is ampli-
fied by PCR.
After one PCR cycle, how many
mole- cules of dsDNA will there be?
molecules of dsDNA after one cycle:
6. After three PCR cycles, how many mol- 8
ecules of dsDNA will there be?
7. After 30 PCR cycles (a typical number of ~1 billion
cycles), how many molecules of
dsDNA will there be?
8. Consider that a typical PCR does not 9. Suppose a scientist is
start with a single molecule of template studying the platypus,
DNA, but rather something in the range one of the few venomous
of 25 nmol of template DNA.
What does this tell you about the
poten- tial of PCR to amplify DNA?
