Histotechnology(questions n answers)graded A+

3 branches of microscopy - correct answers optical, electron, scanning probe 5 steps of sample preparation - correct answers fixation, processing, embedding, sectioning, and staining most common form of microscopy - correct answers bright-field light microscopy 3 types of optical microscopes - correct answers bright-field, phase contrast, fluorescent bright-field microscope - correct answers light microscopy, halogen/LED bulb is light source, compound upright objective turret=most common in labs, must have stained specimen phase contrast microscope - correct answers halogen/LED bulb is light source, found on inverted microscope, image cell cultures, no stain necessary fluorescent microscope - correct answers UV bulb is light source, special objectives available, filter cubes for various wavelengths are required, microscope can have upright or inverted objective turret, plethora of fluorescent probes are available, staining can be antibody or non-antibody format confocal laser scanning probe microscope - correct answers laser is light source, fluorescent imaging only, 3D image is created by scanning multiple focal planes (optical sectioning), very high resolution and contrast, software is critical electron modalities - correct answers scanning electron microscope (SEM) and transmission electron microscope (TEM) scanning electron microscope (SEM) - correct answers light source is electron beam, not the same as confocal laser scanning, creates a 3D topographical image of sample (no stain), sample prep is different than other modalities transmission electron microscopy - correct answers light source is electron beam, can resolve ultrastructure of organelles (black/white image), requires special fixation, processing, embedding, and staining special fixation of TEM uses ______ - correct answers glutaraldehyde special processing and embedding of TEM uses _____ - correct answers resin special staining of TEM uses ______ - correct answers lead fixation - correct answers chemical process that preserves/stabilizes proteins, structures, organelles, etc. based on embedding medium processing - correct answers protocol that dehydrates tissue (removes water) and replaces it with some type of embedding medium, based on embedding medium embedding - correct answers placing the sample in a form and covering with embedding medium, end product is a black, tissue orientation is critical, based on imaging modality sectioning - correct answers cutting the sample block to desired thickness, based on imaging modality staining - correct answers choosing a stain for desired endpoint, specific or general, based on imaging modality most critical step of sample prep - correct answers fixation goal of processing - correct answers to replace fixative with embedding media