ACS Biochemistry 2024 First Semester (Questions with 100% correct answers)

Buffer Order and Henderson Hasselbach correct answers Water, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease Ramachandran plots correct answers Φ = N-R ψ = C-R optimal angle to prevent steric hinderance Protein structure of a helix, B sheet, reverse turn correct answers a: LEMARKHQ B: VICYFTW RT: PSDNG how many amino acids in alpha turn helix correct answers 3.6 Super secondary structure correct answers helix turn helix parallel vs. antiparallel correct answers parallel wedges face same way antiparallel face opposite Isoelectric point (pI) correct answers pH at which a particular molecule carries no net electrical charge. Protein Separation Techniques (ALL) correct answers Homogenization Salting Out Affinity Chromatgraphy Ion Exchange Size Exclusion/Gel Filtration H, S, A, I, SG He said all individuals SinG Protein Separation Techniques (H) correct answers Homogenization disrupt cells, sonicator, centrifuge Protein Separation Techniques (S) correct answers Salting out different proteins precipitate out at different salt concentrations Protein Separation Techniques (A) correct answers Affinity chromatography add a tag; histidine binds; see how well there is an affinity for the group Protein Separation Techniques (I) correct answers ion exchange separation based on charge; + proteins stick to - beads