Protein Technology and Proteome Analysis
Advanced course (mol & cel) 24/01 - 13u30
1) a) You have 2 bacterial strains, one sensitive for antibiotics, one resistant.
You want to know what proteins are involved in the antibiotic resistance. Give
a full flowchart of everything you have to do (all the way from sample
preparation to the identification of your involved proteins).
b) Explain the difference between targeted and untargeted strategies in
metabolomics.
2) a) Give the sequence of the peptide by looking at the graphs (Edman
exercise).
b) What method is used to get this sequence? Give the full mechanism of
action (including chemical reactions).
3) a) Explain why microcolumn chromatography is used and why we use it even
when we have enough sample to do a normal chromatography. Use one or
more formulas to clarify.
b) Determine the sequence of the peptide by using the graph and table below.
Give the b and y series as well.
4) 15 true or false questions, can’t remember them.
5) Questions about the article.
a) How many Daltons are between each peak and why do they label
arginine and lysine?
b) Why do they use SILAC and not iTRAQ?
c) In the article, why do they mention you need a minimum of 2 ratio
counts?
d) How did the authors purify only the secreted proteins and not other
proteins for example in the medium?
Advanced course (mol & cel) 24/01 - 13u30
1) a) You have 2 bacterial strains, one sensitive for antibiotics, one resistant.
You want to know what proteins are involved in the antibiotic resistance. Give
a full flowchart of everything you have to do (all the way from sample
preparation to the identification of your involved proteins).
b) Explain the difference between targeted and untargeted strategies in
metabolomics.
2) a) Give the sequence of the peptide by looking at the graphs (Edman
exercise).
b) What method is used to get this sequence? Give the full mechanism of
action (including chemical reactions).
3) a) Explain why microcolumn chromatography is used and why we use it even
when we have enough sample to do a normal chromatography. Use one or
more formulas to clarify.
b) Determine the sequence of the peptide by using the graph and table below.
Give the b and y series as well.
4) 15 true or false questions, can’t remember them.
5) Questions about the article.
a) How many Daltons are between each peak and why do they label
arginine and lysine?
b) Why do they use SILAC and not iTRAQ?
c) In the article, why do they mention you need a minimum of 2 ratio
counts?
d) How did the authors purify only the secreted proteins and not other
proteins for example in the medium?
