ASCP Molecular Biology Certification Exam | 321 Questions with 100% correct Answers | Verified
Pyrimidine - One carbon ring Cytosine, Thymine, Uracil Purine - Two carbon rings Adenine, Guanine How are nucleotides joined together? - Condensation to form phosphodiester bond What is the function of mRNA? - Carries genetic info out of nucleus Transcript translated to protein What is the function of tRNA? - Carries aa to ribosome Anticodon pairs with codon on mRNA strand What is the function of rRNA? - part of ribosome structure most abundant RNA coordinated coupling of tRNA to mRNA codons Feedback inhibition - Product of pathway is noncompetitive inhibitor Binds to allosteric site to slow down rxn b/c too much product Exonucleases - Degrades nucleic acids by removing one terminal nt at a time Cleaves phosphodiester bond at end of chain 5' -- 3' and 3' -- 5'Endonucleases (Prok) - Restriction enzymes Cleaves phoshpodiester bonds w/i poly-nt chain Recognition site is palindromic sequence Types I-V ORI sites - nt sequence where replication is initiated
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