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Summary OCR A-Level Biology 6.3.5 Gel Electrophoresis

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Revision summary of key knowledge from OCR A-Level Biology 6.3.5 Gel Electrophoresis.

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Gel Electrophoresis
Method
 DNA extracted from a sample
 Endonucleases cut DNA into smaller fragments
 Fragments are loaded into wells in an agarose gel covered in a buffer solution
 A charge is passed through the gel
 DNA is negatively charged so is drawn towards the positive charge
 Larger fragments are heavier so move more slowly
 Form invisible bands which are revealed by radioactive or fluorescent probes which
attach to the modified bases
 Bands are read and analysed by a computer

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Chapter 6.3.5
Uploaded on
June 26, 2023
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Written in
2022/2023
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