Molecular Diagnostics; Exam 2 questions with correct answers
A micoarray is testing for: - Answer a lot of genetic characteristics from one sample Southern Hybridization - Answer extract DNA probe w/ DNA Northern Hybridization - Answer extract RNA probe w/ DNA Western Hybridization - Answer extract proteins probe with antibodies What is the first method that became standard in DNA sequencing? - Answer Sanger method What type of PCR does Sanger Method use? - Answer cycle sequencing PCR Cycle Sequencing PCR requirements - Answer 1. template DNA 2. buffer 3. 1 primer (drives the region we sequence) 4. dNTPs and ddNTPs (the label) 5. Taq DNA Polymerase Sanger procedure; current method - Answer 1. DNA extraction 2. PCR run
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- June 9, 2023
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molecular diagnostics exam 2 questions with corre
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