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Samenvatting BV09GEN Flashcards

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In dit document staan oefenvragen over de tentamen stof van BV09GEN. Alle leerdoelen worden hiermee behandeld.

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June 5, 2023
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2022/2023
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GEN Flashcards




2022-2023

, Wat is het doel van CRISPR-Cas9?




QUESTION




het maken van een dubbelstrengs DNA breuk
om hier mutaties/ deleties mee te kunnen
maken, of om hier een transgeen in te stoppen.




ANSWER

, waar staat CRISPR voor?




QUESTION




Clustered regularly interspaced short palindromic
repeats




ANSWER

, Hoe werkt CRISPR?




QUESTION




1. Vreemd virus DNA wordt geknipt
2. Virus DNA (spacer) wordt
geïmplementeerd tussen de CRISPR
sequentie.
3.Door de CRISPR locus transcriptie
wordt de DNA sequentie omgezet in
Pre-crRNA.
4.Door CRISPR RNA processing word
het pre-crRNA in normale crRNA.

ANSWER
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