BACE Exam practice: Multiple Choice questions with correct answers

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? - Answer (50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. - Answer 1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) digest and provide lanA PCR product and the plasmid with appropriate restriction enzymes 3) use ligase to "paste" LanA into a plasmid 4) transform E. coli with plasmid containing LanA how much tetracycline is needed to prepare a 100 mL of 100 mg /ml tetracycline (w/v)? - Answer (100mL)(100mg/ML) = 10000mg (10g) after performing the PCR reaction with the primers she designed to amplify lanA, the same technician found her PCR reaction contained two distinct bands, one at the expected size of 1200 kilobase pairs, and another unexpected band at 2000 Kilobase pairs. what is the most likely reason there's an unexpected bands and her PCR reaction? - Answer she used an annealing temperature that was too low in a biotechnology lab, tap water is best to use for: - Answer first rinse when washing glassware