BACE written/practical exam - another bace test

after successful bacterial transformation with plasmid DNA, you plan to isolate the plasmid DNA from the genomic DNA using a miniprep. what should you not do? - use water to precipitate and wash DNA how would you describe a tertiary protein structure? - the three dimensional shape of the polypeptide chain once is folded what is true about about protein synthesis? - transfer RNA (tRNA) molecules that carry amino acids you observe an unknown cell under a microscope. a nucleus is visible. what kind of cell is it? - eukaryotic under normal physiological conditions, the cell's plasma membrane prevents exogenous nucleic acids from entering the cell. this is because the membrane is: - impermeable to charged substances what diffuses from the plant shoot tips to cause cell differentiation? - hormone what is the gram stain procedure in order? - crystal violet as stain, iodine as mordant, alcohol as decolorizing agent, gram safranin as counter stain in the ABO blood type system, what is the universal donor? - type O describe macrophage - draw immune cells to infection site, causing inflammation describe t-cells - process and present antigens on their cell surfaces describe b-cells - produce antibodies describe antibodies - bind and label pathogens to start the process of transcription, RNA polymerase binds to the ________________ to initiate mRNA synthesis - operon utilizing a patient's own cells to produce a novel drug or medical treatment is called? - personalized medicine monoclonal antibodies are derived from the fusion of tumor cells and B cells, which are then grown in cell culture. what is this technique called? - hybridoma technology what technique would you use to distinguish a GMO-containing food from a non-GMO-containing food? - PCR golden rice is a rice variety genetically engineered to produce beta-carotene, a precursor of vitamin A. what is not a benefit? - encouraging crop biodiversity what is an ethical use of genetic screening? - developing personalized medical treatments prior to testing in humans, a new drug candidate is tested for safety what regulatory practices? - cGLPs what is correct in regards to clinical trials? - the emphasis of a Phase I clinical trial is on treatment safety When a co-worker wears nitrile groves out of the lab, what is the appropriate action? - notify the co-worker to not wear gloves outside the lab you notice a tear in the packaging of a disposable loop that you are about to use for an experiment. what is the correct response? - discard. the content and packaging is compromised and the risk of contamination is high what is a technique for harvesting bacteria from overnight liquid culture? - streaking when is it acceptable to aseptically transfer bacteria without wearing gloves? - it is never acceptable