AAB Molecular Diagnostics Testing QUESTIONS WITH COMPLETE SOLUTIONS
This DNA amplification method amplifies the nucleic acid used as a probe? correct answer: Ligase Chain Reaction (LCR) What enzymes are used in Ligase Chain Reactions? correct answer: DNA polymerase and DNA ligase What is the function of DNA polymerase in Ligase Chain Reaction? correct answer: Is used to initiate DNA polymerase. What is the key advantage of using Ligase Chain Reaction instead of Polymerase Chain Reaction? correct answer: Better specificity How does Ligase Chain Reaction differ from Polymerase Chain Reaction? correct answer: This method uses a thermostable ligase to join to probes which are then amplified by standard PCR cycling. Why was Ligase Chain Reaction originally developed? correct answer: To detect point mutation.
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aab molecular diagnostics testing questions with complete solutions
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