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Summary

Volledige samenvatting VCNW structuur

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Uploaded on
23-02-2023
Written in
2021/2022

Een redelijk beknopte maar wel totaal volledige samenvatting van het vak VCNW structuur (1e Bach). Als je dit document kent, samen met de afbeeldingen in de slides, haal je gegarandeerd fantastische punten op het examen. Ik heb zelf van de eerste keer een 16/20 gehaald, door dit document te maken en te gebruiken.

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Institution
Katholieke Universiteit Leuven (KU Leuven)
Course
Van Cel Naar Weefsel: Structuur (BKULE04Y4A)

Content preview

H1: methoden & technieken voor microscopie
1) Staalafname
a) Biopsie
Endoscopisch
CNB
Punch
Excisie / incisie
b) Afzonderlijke cellen
FNAC
Vocht
Cervixbrush
Bloed- / beenmerguitstrijkje
2) Voorbereiding
a) Fixatie
Immersie / perfusie in formaldehyde / alcohol
- Stoppen van enzymfuncties
- 1:2
Paraffinecoupes (duurt lang)
Dehydratatie, klaring van alcohol, paraffinering, snijden met
microtoom, deparaffineren
Eventueel IHC
b) Invriezen & snijden met cryostaat
Snel! → preoperatief
Enzymactiviteit bewaard
Mindere kwaliteit
3) Kleuring
a) H&E
- Cytoplasmatische eiwitten & collageen = acidofiel → binden aan zure
eosine → roos
- DNA, RNA, koolhydraten & RER = basofiel → binden aan basische
haematoxyline → paars
b) PAS
Schiff reagens (basisch fuchsine) + aldehyde groepen (KH) → magenta
Glycogeen, mucus, BM, brush borders, reticuline vezels
c) IHC
Antistoffen op zoek naar antigenen (eiwitten) → bruine kleur
Direct / indirect
Monoklonaal / polyklonaal
d) FISH
DNA/RNA seq
e) CISH
Combinatie IHC + ISH

, H3: celinteracties
Metastasering !
Afbraak door MMPs

CEL - ECM
Hemidesmosomen
Cytoskelet → integrines → fibronectine → basale membraan
Basale membraan (basale lamina / lamina externa + reticulaire laag): collageen
IV / elastisch + glycoproteïnen

CEL - CEL
1. Tight junctions (zonula occludens)
Occludines & claudines
Afsluiting
2. Tight junctions (zonula adhaerens)
Cadherines → cateninen → microfilamenten (actine)
Aanhechting
3. Desmosomen (macula adhaerens)
Cadherines → intermediaire filamenten (cytoskelet)
Aanhechting
4. Gap junctions (Nexus)
6 connexinen → connexon
Elektrische en metabolische communicatie
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Institution
Katholieke Universiteit Leuven (KU Leuven)
Study
Geneeskunde
Course
Van Cel Naar Weefsel: Structuur (BKULE04Y4A)

Document information

Uploaded on
February 23, 2023
Number of pages
14
Written in
2021/2022
Type
SUMMARY

Subjects

  • structuur
  • huid
  • kraakbeen
  • bot
  • epitheel
  • cellen
  • cel
  • vcnw
  • bachelor
  • geneeskunde
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