BIOS 242 Week 7 Lab 2; Unknown Bacteria

Lab14:UnknownWorksheet LearningObjectives: • Applyknowledgeacquiredinthelaboratoryexercises • Predicttheidentityofanunknownbacteriainthegivencasestudy • Based on the two dichotomous keys given, answer the following case studies. For each casestudyyoushouldwrite1-2paragraphs,usingcompletesentences,astohowyouarrivedatyour conclusion.YoushouldtakeusstepbystepthroughtheprocessANDindicatehowyouruledoutothermicroorganisms.SimplysayingIusedthedichotomoustreeisn’tsufficient. Case Study 1: Justin was spending the summer with his father on their ranch in Wyoming. One day, as they wereclearing brush and branches from a deserted barn, Justin was struck in the eye with a tree branch. Partof the tree branch penetrated Justin’s eye. The eye continued to worsen over the next few days andJustin’sfathertookhimtotheemergencyroom.Aculturewasperformedwiththeresultsshownbelow.Basedonthedichotomoustree,whatorganismhasinfectedJustin’seye. Case Study 2: Sharon just graduated from high school and her parents took her on a celebration trip to Venezuela. Onthetrip,SharonandherfamilyvisitedmanysmallvillagesandSharonhadtheopportunitytoexperiencelifeinthesevillages.Sharonandherfamilyweretreatedtolocalcuisinemadefromfreshvegetables.Onthe final day of the trip Sharon and her family all came down with an intense case of diarrhea. Lab testswere performed with the results shown below. Based upon the dichotomous chart, what organismcausedthe diarrhea? CaseStudy3: Jennifer was a new nurse at the local hospital. She had recently passed her NCLEX exam and was excitedto be working at her dream job. Jennifer was asked to start an IV on a heart patient so that they couldgive the patient IV medications. She was thrilled for the opportunity to show off her excellent skills atstarting IVs. As Jennifer removed the cap from the needle, she accidentally brushed the needle againsttheskinonherforearm.Jenniferknewthatsheshouldgetanewneedlebutshewasconcernedthatshewould get in trouble so went ahead and used the needle to start the IV. The next day, the heart patientspiked a fever and showed other indications of septic infection. Blood cultures were ordered and theresults are shown below. Using the dichotomous tree, determine the organism that is causing problemsfor the heart patient. CaseStudy4: Juanita was happy and excited about the birth of her new baby boy Joshua. Although Joshua was bornprematurely,hethrivedinthehospitalandwasreleasedtwoweeksafterbirth.Onthefifthdayathome,Juanita noticed that Joshua wasn’t interested in breast feeding and that he seemed lethargic. Juanitabecamemoreconcernedwhenshenoticedredspotsontheblanketsheusedwhenbreastfeeding. Fearingthattherewasamajorproblem,JuanitatookJoshuatothehospital.ThehospitalaskedJuanitafor some expressed breast milk which was sent to the laboratory for culture. The results of the culturearebelow.Basedonthedichotomoustree,whatorganismiscausingproblemsforJoshua? Questions: Inthislabyouusedadichotomoustreetoidentifyyourunknownbacteria.Pleasedescribewhatadichotomoustreeis, howitisused,andtheimportance ofsucha tree.