involves in
inserting a
foricgn gene into an
organisms genome resulting
,
me expression of a new
gene
Recombinant DNA :
DNA that is formed when DNA from two different
sources is joined together
genetic GMO
→
always results in recombinant →
eng
.
Recombinant host :
organism relieving DNA transferred from another organism
for a protein product to be synthesised and harvested
STEPS :
1.
Obtaining me
gene
-
cooing for me desired protein & remove from donor
organism
2.
Cloning of isolated gene of DNA
using
DNA
polymerase PCR
-
3. Transformation -
transfer of DNA into host cell
eg
.
using viral vector .
Identification have
of host cells that
using
4. me
successfully gene
-
taken up
markers
gene
S
large scale production of population of host cells reproduce this
organism on
-
.
,
a
large scale Cfermenters )
inserting a
foricgn gene into an
organisms genome resulting
,
me expression of a new
gene
Recombinant DNA :
DNA that is formed when DNA from two different
sources is joined together
genetic GMO
→
always results in recombinant →
eng
.
Recombinant host :
organism relieving DNA transferred from another organism
for a protein product to be synthesised and harvested
STEPS :
1.
Obtaining me
gene
-
cooing for me desired protein & remove from donor
organism
2.
Cloning of isolated gene of DNA
using
DNA
polymerase PCR
-
3. Transformation -
transfer of DNA into host cell
eg
.
using viral vector .
Identification have
of host cells that
using
4. me
successfully gene
-
taken up
markers
gene
S
large scale production of population of host cells reproduce this
organism on
-
.
,
a
large scale Cfermenters )
