A level Biology AQA Biological molecules

Teachers Factors affecting
blotching enzyme activity
-
.
- regenerating




/
TEMPERATURE
temperature optimum
'

The rate will
get faster as the rises up to the P .




due to the enzymes and substrates having kinetic
energy
-

more

and therefore greater chance of substrate complexes
a
enzyme
-




At higher temperature the enzyme is denatured and stops
•

working
Bonds holding together the tertiary structure of
-




^
enzyme break changes
so the active site shape
E
,



and is no
longer complementary .

j
:

I
.
temp .




PH
the rate of reaction is highest is at the enzymes optimum
•

when the PH
-

At PHS that outside denatured specifically
are the optimum the
enzyme is
+
,



H
-



due to the presence of excess OH or ions which leads
^
"" " " "
"" " " " "" "" " " .




:\
É
of
-




SUBSTRATE CONCENTRATION PH

The rate will faster substrate but will reach
'

get as there is more a


point ,
5 , where all the active sites are full and cannot go
any faster .




^
E .
E
g
÷
É
-

>

Substrate cone


ENZYME CONCENTRATION
-
The rate will get faster there is more and will keep
as
enzyme getting
faster as long as the substrate is in excess

because there is greater chance of Substrate
-


a enzyme
-




complex forming .



A

±
É
:
0



÷
-

>

enzyme cone .