BIOS256 Week 3 Lab #3 Enzyme Activity Laboratory Report.pdf
Effect of Substrate Concentration on Enzyme Activity 1. Dependent Variable: Amount of product used (glucose and fructose 2. Independent Variable: Amount of substrate (sucrose) present 3. Controlled Variables: Temperature, incubation time and PH 4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. Optimal density is measured because it measure the amount of glucose and fructose produced 5. Explain why denatured sucrase was used as a control. Because Denatured Sucrose will not have as much activity as the other sucrose 6. Addition of DNS at the end of the incubation period stopped the reaction by denaturing sucrase. Explain why it is important to denature sucrase before measuring product concentration. In order too decrease enzyme activity RESULTS See Table 1: Effect of pH on Sucrase Activity See Graph: Effect of pH on Sucrase Activity
Document information
- School year
- 4
- Uploaded on
- May 16, 2022
- Number of pages
- 4
- Written in
- 2021/2022
- Type
- Exam (elaborations)
- Contains
- Questions & answers
