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Samenvatting FBT05 - Werken in een analyselab voor voeding

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Het bevat een samengevatte tekst van de practica, casusgestuurde en taakgestuurde lessen

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1 Practica
1.1 Bodems
Bodem Oefening ? Selectief ? Positief ? Negatief
?
Baird Parker S. aureus Selectief Grijs-zwarte Geen
kolonies kolonies
KIA (kligler iron Salmonella, Selectief Gele kleur = Rood
agar) Enterobacteriaceae verzuring;
zwarte bodem =
H2S;
gasproductie
LST-bouillon E. coli Selectief Troebel + Helder
(lauryl sulfate gasproductie
tryptose)
Nutriëntenagar Enterobacterieae Niet-selectief Witachtige Geen
kolonies kolonies
PCA (plate Kiemgetal Niet-selectief Kolonies Geen
count agar) (strogeel) kolonies
Peptonwater Algemeen Niet-selectief
Rappaport Salmonella Selectief Troebel + groen Helder +
broth blauw
SS-agar Salmonella Selectief Salmonella: Andere
(salmonella- Rode kolonies kolonies
shigella) met zwart of geen
centrum groei
Tetrathionaat Salmonella Selectief
TSA (tryptic soy Salmonella Niet-selectief Kolonies Geen
agar) (strogeel) kolonies
TSI (triple Enterobacterieae Selectief Gele kleur = Rood
sugar iron) verzuring;
zwarte bodem =
H2S;
gasproductie
VRBG (violet Enterobacterieae Selectief Roze/paarse Geen
red bile kolonies kolonies
glucose)
XLD-agar Salmonella Selectief Salmonella: Andere
(xylose lysine Rode kolonies kolonies
deoxycholate) met zwart of geen
centrum groei
Oxidasetest Enterobacterieae Roze, dan Kleurloos
blauwpaars en
tenslotte
paarszwart
Bevestigings-
Coagulasetest S. aureus Agglutinatie Niets
testen
Indoltest E. coli Ontstaan van Strogele
wijnrode kleur kleur
na toevoegen
Kovaczreagens

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