SBB UPDATED ACTUAL QUESTIONS AND
CORRECT ANSWERS COMPREHENSIVE
STUDY GUIDE
●● Ankryin
Answer: mediates interaction between Band 3 and underlying rbc
skeleton
attaches to spectrin through interaction with protein 4.2
●● Band 4.1
Answer: mediates interactions between GPC and underlying rbc
skeleton
●● Adducin
Answer: interacts with dimeric Band 3 (associated with GPC)
Important for maintaining membrane stability
●● Spectrin
Answer: major component of underlying skeleton
consists of 2 subunits: alpha & beta
,●● ATP source for RBCs
Answer: 90% from Glycolysis (Embden-Meyerhoff pathway)
10% from pentose phosphate pathway
●● Methemoglobin Reductase Pathway
Answer: necessary to maintain heme iron in ferrous (Fe2+) state
Deficiency causes accumulation of methemoglobin, nonfunctional from
of Hgb
●● Leubering Rapaport Shunt
Answer: permits accumulation of 2,3-DPG
Allows rbc to regulate O2 transport during hypoxia
●● Embden-Meyerhoff Pathway
Answer: Whole anaerobic glycolysis pathway taken by rbc
generates 90% of ATP needed for rbc
●● "Tense form" of Hgb
Answer: DEOXYGENATED
widening of beta-chain
lower affinity for O2
expels 2,3-DPG
,●● "Relaxed form" of Hgb
Answer: OXYGENATED
beta-chain pulled together
higher affinity for O2
expels 2,3-DPG
●● "Shift Right"
Answer: higher P50 value
decreased pH (increased H+ ion), acidosis
increased 2,3-DPG
higher temperature
high pCO2
Seen in compensated anemia
●● "Shift Left"
Answer: lower P50 value
increased pH (decresed H+ ion), alkalosis
decreasede 2,3-DPG
lower temeratures
low pCO2
, Seen in storage of RBC
●● Compensated Anemia physiologic reactions
Answer: Increased:
-Cardiac output (heart rate, stroke volume)
- Blood flow (vasodilation, shunting)
- O2 extraction
- O2- Hgb affinity (shift right)
●● Dyserythropoiesis
Answer: HEMPAS, sideroblastic anemia, megaloblastic anemia
Altered erythropoesis quantitatively and qualitatively
increased i
associated with Hypoglycosylation
●● Myeloproliferative Disease
Answer: RBC demonstrate increased adhesion to laminin
-increased expression & phosporylation of Lutheran
Can demonstrate Rh mosaicism
-Tn polyagg is form of mosaicism
CORRECT ANSWERS COMPREHENSIVE
STUDY GUIDE
●● Ankryin
Answer: mediates interaction between Band 3 and underlying rbc
skeleton
attaches to spectrin through interaction with protein 4.2
●● Band 4.1
Answer: mediates interactions between GPC and underlying rbc
skeleton
●● Adducin
Answer: interacts with dimeric Band 3 (associated with GPC)
Important for maintaining membrane stability
●● Spectrin
Answer: major component of underlying skeleton
consists of 2 subunits: alpha & beta
,●● ATP source for RBCs
Answer: 90% from Glycolysis (Embden-Meyerhoff pathway)
10% from pentose phosphate pathway
●● Methemoglobin Reductase Pathway
Answer: necessary to maintain heme iron in ferrous (Fe2+) state
Deficiency causes accumulation of methemoglobin, nonfunctional from
of Hgb
●● Leubering Rapaport Shunt
Answer: permits accumulation of 2,3-DPG
Allows rbc to regulate O2 transport during hypoxia
●● Embden-Meyerhoff Pathway
Answer: Whole anaerobic glycolysis pathway taken by rbc
generates 90% of ATP needed for rbc
●● "Tense form" of Hgb
Answer: DEOXYGENATED
widening of beta-chain
lower affinity for O2
expels 2,3-DPG
,●● "Relaxed form" of Hgb
Answer: OXYGENATED
beta-chain pulled together
higher affinity for O2
expels 2,3-DPG
●● "Shift Right"
Answer: higher P50 value
decreased pH (increased H+ ion), acidosis
increased 2,3-DPG
higher temperature
high pCO2
Seen in compensated anemia
●● "Shift Left"
Answer: lower P50 value
increased pH (decresed H+ ion), alkalosis
decreasede 2,3-DPG
lower temeratures
low pCO2
, Seen in storage of RBC
●● Compensated Anemia physiologic reactions
Answer: Increased:
-Cardiac output (heart rate, stroke volume)
- Blood flow (vasodilation, shunting)
- O2 extraction
- O2- Hgb affinity (shift right)
●● Dyserythropoiesis
Answer: HEMPAS, sideroblastic anemia, megaloblastic anemia
Altered erythropoesis quantitatively and qualitatively
increased i
associated with Hypoglycosylation
●● Myeloproliferative Disease
Answer: RBC demonstrate increased adhesion to laminin
-increased expression & phosporylation of Lutheran
Can demonstrate Rh mosaicism
-Tn polyagg is form of mosaicism
