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BIOD1 of
171210/16/2026,
Subject Chemistry
5:42:03Enzymatic
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Learning
Assays10/16/2026,
BIOD 171 Subject
5:42:03
Chemistry
AM*&!#%#&&!%%#^#^&^!#&*^&^*#!(FG*T!^&*#*((*!#&()#_)#!*&$!*#)(!*##(*0Portage
Enzymatic Assays.pdf*&!#%#&&!%%#^#^&^!#&*^&^*#!(FG*T!^&*#*((*!#&()#_)#!
Learning BIOD 171 Subject Chemistry Enzymatic
Learning
As
RY06 12/16/2024
Enzymatic Assays
Objective - Profile bacterial populations based on key enzymatic reactions and determine
growth/metabolic characteristics.
Notes:
Oxidase Test
Qualitative test to determine the presence/absence of cytochrome c oxidase activity in bacteria
Procedures
● Use a sterile loop to pick an isolated colony from BAP (blood agar plate.)
● Smear directly onto the reaction area of the slide.
● Examine test area for color change within 20 second
Oxidase Negative = No color change
Oxidase Positive = Purple Color
Catalase Test
Qualitative test to determine the presence/absence of catalase, an enzyme used to break down
hydrogen peroxide
Procedures
● Using a sterile loop carefully pick colonies from the plate.
● Smear colony directly onto the glass slide.
● Add 2 drops of hydrogen peroxide to each smear
Catalase Negative = no bubbles
Catalase Positive = bubbles
Coagulase Test
Qualitative test to determine the presence/absence of coagulase, an enzyme that plays a role in
the formation of blood clots.
Procedures
● Using a sterile loop carefully pick colonies from the plate.
● Add colony to tube of rabbit plasma, incubate overnight at 37 ℃.
● Next day examine sample for precipitants (cloudy)
This study source was downloaded by 1827175 from cliffsnotes.com on 01-08-2026 16:10:02 GMT -06:00
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BIOD 1 of
171210/16/2026,
Subject Chemistry
5:42:03Enzymatic
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Learning
Assays10/16/2026,
BIOD 171 Subject
https://www.cliffsnotes.com//study-notes/23217552 5:42:03
Chemistry
AM*&!#%#&&!%%#^#^&^!#&*^&^*#!(FG*T!^&*#*((*!#&()#_)#!*&$!*#)(!*##(*0Portage
Enzymatic Assays.pdf*&!#%#&&!%%#^#^&^!#&*^&^*#!(FG*T!^&*#*((*!#&()#_)#!
Learning BIOD 171 Subject Chemistry Enzymatic
Learning
As
e Learning
Page
BIOD1 of
171210/16/2026,
Subject Chemistry
5:42:03Enzymatic
AM*&!#%#&&!%%#^#^&^!#&*^&^*#!(FG*T!^&*#*((*!#&()#_)#!*&$!*#)(!*Portage
Learning
Assays10/16/2026,
BIOD 171 Subject
5:42:03
Chemistry
AM*&!#%#&&!%%#^#^&^!#&*^&^*#!(FG*T!^&*#*((*!#&()#_)#!*&$!*#)(!*##(*0Portage
Enzymatic Assays.pdf*&!#%#&&!%%#^#^&^!#&*^&^*#!(FG*T!^&*#*((*!#&()#_)#!
Learning BIOD 171 Subject Chemistry Enzymatic
Learning
As
RY06 12/16/2024
Enzymatic Assays
Objective - Profile bacterial populations based on key enzymatic reactions and determine
growth/metabolic characteristics.
Notes:
Oxidase Test
Qualitative test to determine the presence/absence of cytochrome c oxidase activity in bacteria
Procedures
● Use a sterile loop to pick an isolated colony from BAP (blood agar plate.)
● Smear directly onto the reaction area of the slide.
● Examine test area for color change within 20 second
Oxidase Negative = No color change
Oxidase Positive = Purple Color
Catalase Test
Qualitative test to determine the presence/absence of catalase, an enzyme used to break down
hydrogen peroxide
Procedures
● Using a sterile loop carefully pick colonies from the plate.
● Smear colony directly onto the glass slide.
● Add 2 drops of hydrogen peroxide to each smear
Catalase Negative = no bubbles
Catalase Positive = bubbles
Coagulase Test
Qualitative test to determine the presence/absence of coagulase, an enzyme that plays a role in
the formation of blood clots.
Procedures
● Using a sterile loop carefully pick colonies from the plate.
● Add colony to tube of rabbit plasma, incubate overnight at 37 ℃.
● Next day examine sample for precipitants (cloudy)
This study source was downloaded by 1827175 from cliffsnotes.com on 01-08-2026 16:10:02 GMT -06:00
G*T!^&*#*((*!#&()#_)#!*&$!*#)(!*##(*0Portage
e Learning
Page
BIOD 1 of
171210/16/2026,
Subject Chemistry
5:42:03Enzymatic
AM*&!#%#&&!%%#^#^&^!#&*^&^*#!(FG*T!^&*#*((*!#&()#_)#!*&$!*#)(!*Portage
Learning
Assays10/16/2026,
BIOD 171 Subject
https://www.cliffsnotes.com//study-notes/23217552 5:42:03
Chemistry
AM*&!#%#&&!%%#^#^&^!#&*^&^*#!(FG*T!^&*#*((*!#&()#_)#!*&$!*#)(!*##(*0Portage
Enzymatic Assays.pdf*&!#%#&&!%%#^#^&^!#&*^&^*#!(FG*T!^&*#*((*!#&()#_)#!
Learning BIOD 171 Subject Chemistry Enzymatic
Learning
As
