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Microbiology Harr
The aseptic collection of blood cultures requires that the skin be cleansed with:
A. 2% iodin and then 70% alcohol solution
B. 70% alcohol and then 2% iodine or an iodophor
C. 70% alcohol and then 95% alcohol
D. 95% alcohol only - ANSWER-Correct Answer: B
When cleansing the skin with alcohol and then iodine for the collection of a blood
culture, the iodine (or iodophor) should remain intact on the skin for at least:
A. 10 sec
B. 30 sec
C. 60 sec
D. 5 min - ANSWER-Correct Answer: C
What is the purpose of adding 0.025%-0.050% sodium polyanetholsulfanate
(SPS) to nutrient broth media for the collection of blood cultures?
A. It inhibits phagocytosis and complement
B. It promotes formation of a blood clot
C. It enhances growth of anaerobes
D. It functions as a preservative - ANSWER-Correct Answer: A
A flexible calcium alginate nasopharyngeal swab in the collection device of
choice for recovery of which organism from the nasopharynx?
A. Staphylococcus aureus
B. Streptococcus pneumoniae
C. Corynebacterium diphtheriae
D. Bacteroides fragilis - ANSWER-Correct Answer: C
Semisolid transport media such as Amies, Stuart, or Cary-Blair are suitable for
the transport of swabs for culture of most pathogen except:
A. Neisseria gonorrhoeae
B. Enterobacteriaceae
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C. Camplyobacter fetus
D. Streptococcus pneumoniae - ANSWER-Correct Answer: A
Select the method of choice for recovery of anaerobic bacteria from a deep
abscess.
A. Cotton fiver swab or the abscess area
B. Skin snip of the surface tissue
C. Needle aspirate after surface decontamination
D. Swab of the scalpel used for debridement - ANSWER-Correct Answer: C
Select the primary and differential media of choice for recovery of most fecal
pathogens.
A. MacConkey, blood, birdseed, and Campylobacter (Campy) agars
B. Hektoen, MacConkey, Campy, colistin-nalidixic acid (CNA) agars
C. CNA and Christensen urea agars and thioglycollate media
D. Blood, Campy, Mueller-Hinton agars, and thioglycollate media -
ANSWER-Correct Answer: B
Select the media of choice for recovery of Vibrio cholerae form a stool specimen.
A. MacConkey agar and thioglycollate media
B. Thiosulfate-citrate-bile sucrose (TCBS_ agar and alkaline peptone water
(APW broth)
C. Blood agar and selenite-F (SEL) broth
D. CNA agar - ANSWER-Correct Answer: B
Colistin-nalidixic acid agar (CNA) is used primarily for the recovery of:
A. Neisseria species
B. Enterobacteriaceae
C. Pseudomonas aeruginosa
D. Staphylococcus - ANSWER-Correct Answer: D
In the United States, most blood agar plates are prepared with 5% or 10% red
blood cells (RBCs) obtained from:
A. Sheep
B. Horses
C. Humans
D. Dogs - ANSWER-Correct Answer: A
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All of the following are appropriate when attempting to isolate N. gonorrheae from
a genital specimen except:
A. Transport the genital swab in charcoal transport medium
B. Plate the specimen on modified Thayer-Marin (MTM) medium
C. Plate the specimen on New York City or Martin-Lewis agar
D. Culture specimens in ambient oxygen at 37C - ANSWER-Correct Answer: D
Chocolate agar and modified Thayer-Martin agar are used for the recovery of:
A. Haemophilus spp. and Neisseria spp., respectively
B. Haemophilus spp. and N. gonorrhoeae, respectively
C. Neisseria spp. and Streptococcus spp., respectively
D. Streptococcus spp. and Staphylococcus spp., respectively - ANSWER-Correct
Answer: B
Cycloserine-cefoxitin-fructose agar (CCFA) is used for the recovery of:
A. Yersinia enterocolitica
B. Yersinia intermedia
C. Clostridium perfringens
D. Clostridium difficile - ANSWER-Correct Answer: D
Deoxycholate agar (DCA) is useful for the isolation of:
A. Enterobacteriaceae
B. Enterococcus spp.
C. Staphylococcus spp.
D. Neisseria spp. - ANSWER-Correct Answer: A
Xylose lysine deoxycholate (XLD) agar is a highly selective medium used for the
recovery of which bacteria?
A. Staphylococcus spp. from normal flora
B. Yersinia spp. that do not grow on Hektoen agar
C. Enterobacteriaceae from gastrointestinal specimens
D. Streptococcus spp. from stool cultures - ANSWER-Correct Answer: C
A sheep blood agar plate is used as a primary isolation medium when all of the
following organisms are to be recovered from a wound specimen except:
A. Hemolytic streptococci and coagulase-positive staphylococci
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B. Haemophilus influenzae and Haemophilus parainfluenzae
C. Proteus spp. and Escherichia coli
D. Pseudomonas spp. and Acinetobacter spp. - ANSWER-Correct Answer: B
Prereduced and vitamin K1-supplemented blood agar plates are recommended
isolation media for:
A. Mycobacterium marinum and Mycobacterium avium intracellulare
B. Bacteroides, Peptostreptococcus, and Clostridium spp.
C. Proteus spp.
D. Enterococcus spp. - ANSWER-Correct Answer: B
Which procedure is appropriate for culture of genital specimens in order to
recover Chlamydia spp.?
A. Inoculate cycloheximide-treated McCoy cells
B. Plate onto blood and chocolate agar
C. Inoculate into thioglycollate (THIO) broth
D. Plate onto modified Thayer-Martin agar within 24 hours - ANSWER-Correct
Answer: A
Specimens for virus culture should be transported in media containing:
A. Antibiotics and 5% sheep blood
B. Saline and 5% sheep blood
C. 22% bovine albumin
D. Antibiotics and nutrient - ANSWER-Correct Answer: D
Cerebrospinal fluid (CSF) should be cultured immediately, but if delayed the
specimen should be:
A. Refrigerated at 4C to 6C
B. Frozen at -20C
C. Stored at room temperature for no longer than 24 hours
D. Incubated at 37C and cultured as soon as possible - ANSWER-Correct
Answer: D
The most sensitive method for the detection of B-lactamase in bacteria is by the
use of:
A. Chromogenic cephalosporin
B. Penicillin
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Microbiology Harr
The aseptic collection of blood cultures requires that the skin be cleansed with:
A. 2% iodin and then 70% alcohol solution
B. 70% alcohol and then 2% iodine or an iodophor
C. 70% alcohol and then 95% alcohol
D. 95% alcohol only - ANSWER-Correct Answer: B
When cleansing the skin with alcohol and then iodine for the collection of a blood
culture, the iodine (or iodophor) should remain intact on the skin for at least:
A. 10 sec
B. 30 sec
C. 60 sec
D. 5 min - ANSWER-Correct Answer: C
What is the purpose of adding 0.025%-0.050% sodium polyanetholsulfanate
(SPS) to nutrient broth media for the collection of blood cultures?
A. It inhibits phagocytosis and complement
B. It promotes formation of a blood clot
C. It enhances growth of anaerobes
D. It functions as a preservative - ANSWER-Correct Answer: A
A flexible calcium alginate nasopharyngeal swab in the collection device of
choice for recovery of which organism from the nasopharynx?
A. Staphylococcus aureus
B. Streptococcus pneumoniae
C. Corynebacterium diphtheriae
D. Bacteroides fragilis - ANSWER-Correct Answer: C
Semisolid transport media such as Amies, Stuart, or Cary-Blair are suitable for
the transport of swabs for culture of most pathogen except:
A. Neisseria gonorrhoeae
B. Enterobacteriaceae
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C. Camplyobacter fetus
D. Streptococcus pneumoniae - ANSWER-Correct Answer: A
Select the method of choice for recovery of anaerobic bacteria from a deep
abscess.
A. Cotton fiver swab or the abscess area
B. Skin snip of the surface tissue
C. Needle aspirate after surface decontamination
D. Swab of the scalpel used for debridement - ANSWER-Correct Answer: C
Select the primary and differential media of choice for recovery of most fecal
pathogens.
A. MacConkey, blood, birdseed, and Campylobacter (Campy) agars
B. Hektoen, MacConkey, Campy, colistin-nalidixic acid (CNA) agars
C. CNA and Christensen urea agars and thioglycollate media
D. Blood, Campy, Mueller-Hinton agars, and thioglycollate media -
ANSWER-Correct Answer: B
Select the media of choice for recovery of Vibrio cholerae form a stool specimen.
A. MacConkey agar and thioglycollate media
B. Thiosulfate-citrate-bile sucrose (TCBS_ agar and alkaline peptone water
(APW broth)
C. Blood agar and selenite-F (SEL) broth
D. CNA agar - ANSWER-Correct Answer: B
Colistin-nalidixic acid agar (CNA) is used primarily for the recovery of:
A. Neisseria species
B. Enterobacteriaceae
C. Pseudomonas aeruginosa
D. Staphylococcus - ANSWER-Correct Answer: D
In the United States, most blood agar plates are prepared with 5% or 10% red
blood cells (RBCs) obtained from:
A. Sheep
B. Horses
C. Humans
D. Dogs - ANSWER-Correct Answer: A
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All of the following are appropriate when attempting to isolate N. gonorrheae from
a genital specimen except:
A. Transport the genital swab in charcoal transport medium
B. Plate the specimen on modified Thayer-Marin (MTM) medium
C. Plate the specimen on New York City or Martin-Lewis agar
D. Culture specimens in ambient oxygen at 37C - ANSWER-Correct Answer: D
Chocolate agar and modified Thayer-Martin agar are used for the recovery of:
A. Haemophilus spp. and Neisseria spp., respectively
B. Haemophilus spp. and N. gonorrhoeae, respectively
C. Neisseria spp. and Streptococcus spp., respectively
D. Streptococcus spp. and Staphylococcus spp., respectively - ANSWER-Correct
Answer: B
Cycloserine-cefoxitin-fructose agar (CCFA) is used for the recovery of:
A. Yersinia enterocolitica
B. Yersinia intermedia
C. Clostridium perfringens
D. Clostridium difficile - ANSWER-Correct Answer: D
Deoxycholate agar (DCA) is useful for the isolation of:
A. Enterobacteriaceae
B. Enterococcus spp.
C. Staphylococcus spp.
D. Neisseria spp. - ANSWER-Correct Answer: A
Xylose lysine deoxycholate (XLD) agar is a highly selective medium used for the
recovery of which bacteria?
A. Staphylococcus spp. from normal flora
B. Yersinia spp. that do not grow on Hektoen agar
C. Enterobacteriaceae from gastrointestinal specimens
D. Streptococcus spp. from stool cultures - ANSWER-Correct Answer: C
A sheep blood agar plate is used as a primary isolation medium when all of the
following organisms are to be recovered from a wound specimen except:
A. Hemolytic streptococci and coagulase-positive staphylococci
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B. Haemophilus influenzae and Haemophilus parainfluenzae
C. Proteus spp. and Escherichia coli
D. Pseudomonas spp. and Acinetobacter spp. - ANSWER-Correct Answer: B
Prereduced and vitamin K1-supplemented blood agar plates are recommended
isolation media for:
A. Mycobacterium marinum and Mycobacterium avium intracellulare
B. Bacteroides, Peptostreptococcus, and Clostridium spp.
C. Proteus spp.
D. Enterococcus spp. - ANSWER-Correct Answer: B
Which procedure is appropriate for culture of genital specimens in order to
recover Chlamydia spp.?
A. Inoculate cycloheximide-treated McCoy cells
B. Plate onto blood and chocolate agar
C. Inoculate into thioglycollate (THIO) broth
D. Plate onto modified Thayer-Martin agar within 24 hours - ANSWER-Correct
Answer: A
Specimens for virus culture should be transported in media containing:
A. Antibiotics and 5% sheep blood
B. Saline and 5% sheep blood
C. 22% bovine albumin
D. Antibiotics and nutrient - ANSWER-Correct Answer: D
Cerebrospinal fluid (CSF) should be cultured immediately, but if delayed the
specimen should be:
A. Refrigerated at 4C to 6C
B. Frozen at -20C
C. Stored at room temperature for no longer than 24 hours
D. Incubated at 37C and cultured as soon as possible - ANSWER-Correct
Answer: D
The most sensitive method for the detection of B-lactamase in bacteria is by the
use of:
A. Chromogenic cephalosporin
B. Penicillin
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