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BIOD171 Microbiology Lab 2026/2027 | Lab Notebook, Study Guide & Practical Exam Review

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BIOD171 Microbiology Lab 2026/2027 | Lab Notebook, Study Guide & Practical Exam Review

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Lab Notebook10/16/2026, 5:42:03
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Portage Learning
BIO 171- Microbiology
Lab Notebook

Lab Notebook Bookmarks (click to navigate):

Lab 1 Notebook

Lab 2 Notebook

Lab 3 Notebook

Lab 4 Notebook

Lab 5 Notebook

Lab 6 Notebook

Lab 7 Notebook

Lab 8 Notebook

Lab 9 Notebook




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Microbiology Microbiology Lab Notebook
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Lab 1 Notebook
Back to Home Page




Title: JG01 Introduction to Medical Microbiology


Objective:
To learn lab safety, how to maintain a lab notebook as well as identification and
function of general lab equipment.


Cultivation of samples (growth conditions) - equipment used
Identification of samples (biochemical assays) - tests available
Evaluation of samples (microscopy) - visualization and recognition of key characteristics


Procedure:
Basic Equipment
Cleaning
● Autoclave
○ Heats up to 125℃
○ Uses heat, pressure and steam to sterilize the environment.
○ Steam is more efficient to sterilize an environment compared to dry air.
Growing
● Fixed incubator
○ Maintained around 37℃
○ Able to hold petri dishes as well as tubes for liquid cultures.
○ Door is air tight and also can be covered by a metal door to control the light
conditions throughout the experiment
● Shaker incubator
○ Maintained around 37℃
○ Utilized when using a growth media, a liquid nutrient broth, to inoculate
bacteria.
○ Shakes the culture in a swirling fashion to help aerate the bacteria,which in turn
helps it to grow




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Visualizing
● Microscopy
Storing
● Refrigerator
○ Maintained around 4℃
○ This temperature slows the bacteria's growth to preserve the samples that are
being tested
○ Used when a study cannot be completed in one day
Lab Safety
● Never eat or drink in the lab
○ contamination risks
● Use Personal Protective Equipment (PPE)
○ latex or thermal gloves (application dependent)
○ eyewear protection
○ lab coat
● Never leave the lab while wearing PPE
○ public hallway, bathroom, cafeteria
Lab Notebook
Title: First Name, Last Name Initial Exp# Title of Experiment
Objective: Display purpose of the experiment
Procedure: Each step of a given protocol is recorded
● Temperature/chemical used/growth conditions
● Clearly indicate any deviations that occur during experiment
Notes: Additional information placed here.
Results: A summary of final outcome of the experiment


Notes: Remember to use lab notebook to take notes while watching lab videos.


Results: Learned some functions of general lab equipment, general lab safety, and how to
maintain a lab notebook.




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