, BIOCHEMISTRY
Metabolism I - Carbohydrates & Bioenergetics - Chapters 7-12 Practice Questions 2026/2027
Edition
Original Practice Questions - For Study Purposes
,Q1. Which statement about Glycolysis overall 10 steps glucose to 2 pyruvate net 2 ATP 2 NADH
location cytosol is correct?
A. Incorrect: Misstates Glycolysis overall 10 steps glucose to 2 pyruvate net 2 ATP 2 NADH location cytosol.
B. Correct: Glycolysis overall 10 steps glucose to 2 pyruvate net 2 ATP 2 NADH location cytosol as defined in
Campbell 10th Edition - accurate mechanism, regulation, and physiological relevance.
C. Incorrect: Confuses Glycolysis overall 10 steps glucose to 2 pyruvate net 2 ATP 2 NADH location cytosol
with unrelated pathway.
D. Incorrect: Claims Glycolysis overall 10 steps glucose to 2 pyruvate net 2 ATP 2 NADH location cytosol does
not occur or has no function.
Correct: B | Rationale: Glycolysis overall 10 steps glucose to 2 pyruvate net 2 ATP 2 NADH location cytosol:
Validated against Campbell Biochemistry 10th Ed Chapters 7-12. Unique concept not repeated elsewhere.
Q2. Which statement about Glycolysis step 1 hexokinase glucose to glucose-6-P ATP inhibited
by G6P is correct?
A. Incorrect: Misstates Glycolysis step 1 hexokinase glucose to glucose-6-P ATP inhibited by G6P.
B. Incorrect: Confuses Glycolysis step 1 hexokinase glucose to glucose-6-P ATP inhibited by G6P with
unrelated pathway.
C. Correct: Glycolysis step 1 hexokinase glucose to glucose-6-P ATP inhibited by G6P as defined in Campbell
10th Edition - accurate mechanism, regulation, and physiological relevance.
D. Incorrect: Claims Glycolysis step 1 hexokinase glucose to glucose-6-P ATP inhibited by G6P does not occur
or has no function.
Correct: C | Rationale: Glycolysis step 1 hexokinase glucose to glucose-6-P ATP inhibited by G6P: Validated
against Campbell Biochemistry 10th Ed Chapters 7-12. Unique concept not repeated elsewhere.
Q3. Which statement about Glycolysis step 2 phosphoglucose isomerase G6P to fructose-6-P
is correct?
A. Incorrect: Misstates Glycolysis step 2 phosphoglucose isomerase G6P to fructose-6-P.
B. Incorrect: Claims Glycolysis step 2 phosphoglucose isomerase G6P to fructose-6-P does not occur or has
no function.
C. Incorrect: Confuses Glycolysis step 2 phosphoglucose isomerase G6P to fructose-6-P with unrelated
pathway.
D. Correct: Glycolysis step 2 phosphoglucose isomerase G6P to fructose-6-P as defined in Campbell 10th
Edition - accurate mechanism, regulation, and physiological relevance.
Correct: D | Rationale: Glycolysis step 2 phosphoglucose isomerase G6P to fructose-6-P: Validated against
Campbell Biochemistry 10th Ed Chapters 7-12. Unique concept not repeated elsewhere.
Q4. Which statement about Glycolysis step 3 phosphofructokinase-1 PFK-1 fructose-6-P to
fructose-1,6-bisP rate limiting regulated ATP ADP AMP F2,6BP is correct?
A. Correct: Glycolysis step 3 phosphofructokinase-1 PFK-1 fructose-6-P to fructose-1,6-bisP rate limiting
regulated ATP ADP AMP F2,6BP as defined in Campbell 10th Edition - accurate mechanism, regulation, and
physiological relevance.
B. Incorrect: Misstates Glycolysis step 3 phosphofructokinase-1 PFK-1 fructose-6-P to fructose-1,6-bisP rate
limiting regulated ATP ADP AMP F2,6BP.
C. Incorrect: Confuses Glycolysis step 3 phosphofructokinase-1 PFK-1 fructose-6-P to fructose-1,6-bisP rate
limiting regulated ATP ADP AMP F2,6BP with unrelated pathway.
D. Incorrect: Claims Glycolysis step 3 phosphofructokinase-1 PFK-1 fructose-6-P to fructose-1,6-bisP rate
limiting regulated ATP ADP AMP F2,6BP does not occur or has no function.
Correct: A | Rationale: Glycolysis step 3 phosphofructokinase-1 PFK-1 fructose-6-P to fructose-1,6-bisP rate
limiting regulated ATP ADP AMP F2,6BP: Validated against Campbell Biochemistry 10th Ed Chapters 7-12.
Unique concept not repeated elsewhere.
, Q5. Which statement about PFK-1 regulation ATP inhibitor ADP AMP activator citrate inhibitor
pH is correct?
A. Incorrect: Misstates PFK-1 regulation ATP inhibitor ADP AMP activator citrate inhibitor pH.
B. Correct: PFK-1 regulation ATP inhibitor ADP AMP activator citrate inhibitor pH as defined in Campbell 10th
Edition - accurate mechanism, regulation, and physiological relevance.
C. Incorrect: Confuses PFK-1 regulation ATP inhibitor ADP AMP activator citrate inhibitor pH with unrelated
pathway.
D. Incorrect: Claims PFK-1 regulation ATP inhibitor ADP AMP activator citrate inhibitor pH does not occur or
has no function.
Correct: B | Rationale: PFK-1 regulation ATP inhibitor ADP AMP activator citrate inhibitor pH: Validated
against Campbell Biochemistry 10th Ed Chapters 7-12. Unique concept not repeated elsewhere.
Q6. Which statement about Fructose 2,6-bisphosphate PFK-2/FBPase-2 bifunctional regulation
insulin glucagon is correct?
A. Incorrect: Misstates Fructose 2,6-bisphosphate PFK-2/FBPase-2 bifunctional regulation insulin glucagon.
B. Incorrect: Confuses Fructose 2,6-bisphosphate PFK-2/FBPase-2 bifunctional regulation insulin glucagon
with unrelated pathway.
C. Correct: Fructose 2,6-bisphosphate PFK-2/FBPase-2 bifunctional regulation insulin glucagon as defined in
Campbell 10th Edition - accurate mechanism, regulation, and physiological relevance.
D. Incorrect: Claims Fructose 2,6-bisphosphate PFK-2/FBPase-2 bifunctional regulation insulin glucagon does
not occur or has no function.
Correct: C | Rationale: Fructose 2,6-bisphosphate PFK-2/FBPase-2 bifunctional regulation insulin glucagon:
Validated against Campbell Biochemistry 10th Ed Chapters 7-12. Unique concept not repeated elsewhere.
Q7. Which statement about Glycolysis step 4 aldolase fructose-1,6-bisP to GAP and DHAP is
correct?
A. Incorrect: Misstates Glycolysis step 4 aldolase fructose-1,6-bisP to GAP and DHAP.
B. Incorrect: Claims Glycolysis step 4 aldolase fructose-1,6-bisP to GAP and DHAP does not occur or has no
function.
C. Incorrect: Confuses Glycolysis step 4 aldolase fructose-1,6-bisP to GAP and DHAP with unrelated pathway.
D. Correct: Glycolysis step 4 aldolase fructose-1,6-bisP to GAP and DHAP as defined in Campbell 10th Edition
- accurate mechanism, regulation, and physiological relevance.
Correct: D | Rationale: Glycolysis step 4 aldolase fructose-1,6-bisP to GAP and DHAP: Validated against
Campbell Biochemistry 10th Ed Chapters 7-12. Unique concept not repeated elsewhere.
Q8. Which statement about Glycolysis step 5 triose phosphate isomerase TIM DHAP to GAP is
correct?
A. Correct: Glycolysis step 5 triose phosphate isomerase TIM DHAP to GAP as defined in Campbell 10th
Edition - accurate mechanism, regulation, and physiological relevance.
B. Incorrect: Misstates Glycolysis step 5 triose phosphate isomerase TIM DHAP to GAP.
C. Incorrect: Confuses Glycolysis step 5 triose phosphate isomerase TIM DHAP to GAP with unrelated
pathway.
D. Incorrect: Claims Glycolysis step 5 triose phosphate isomerase TIM DHAP to GAP does not occur or has no
function.
Correct: A | Rationale: Glycolysis step 5 triose phosphate isomerase TIM DHAP to GAP: Validated against
Campbell Biochemistry 10th Ed Chapters 7-12. Unique concept not repeated elsewhere.
Metabolism I - Carbohydrates & Bioenergetics - Chapters 7-12 Practice Questions 2026/2027
Edition
Original Practice Questions - For Study Purposes
,Q1. Which statement about Glycolysis overall 10 steps glucose to 2 pyruvate net 2 ATP 2 NADH
location cytosol is correct?
A. Incorrect: Misstates Glycolysis overall 10 steps glucose to 2 pyruvate net 2 ATP 2 NADH location cytosol.
B. Correct: Glycolysis overall 10 steps glucose to 2 pyruvate net 2 ATP 2 NADH location cytosol as defined in
Campbell 10th Edition - accurate mechanism, regulation, and physiological relevance.
C. Incorrect: Confuses Glycolysis overall 10 steps glucose to 2 pyruvate net 2 ATP 2 NADH location cytosol
with unrelated pathway.
D. Incorrect: Claims Glycolysis overall 10 steps glucose to 2 pyruvate net 2 ATP 2 NADH location cytosol does
not occur or has no function.
Correct: B | Rationale: Glycolysis overall 10 steps glucose to 2 pyruvate net 2 ATP 2 NADH location cytosol:
Validated against Campbell Biochemistry 10th Ed Chapters 7-12. Unique concept not repeated elsewhere.
Q2. Which statement about Glycolysis step 1 hexokinase glucose to glucose-6-P ATP inhibited
by G6P is correct?
A. Incorrect: Misstates Glycolysis step 1 hexokinase glucose to glucose-6-P ATP inhibited by G6P.
B. Incorrect: Confuses Glycolysis step 1 hexokinase glucose to glucose-6-P ATP inhibited by G6P with
unrelated pathway.
C. Correct: Glycolysis step 1 hexokinase glucose to glucose-6-P ATP inhibited by G6P as defined in Campbell
10th Edition - accurate mechanism, regulation, and physiological relevance.
D. Incorrect: Claims Glycolysis step 1 hexokinase glucose to glucose-6-P ATP inhibited by G6P does not occur
or has no function.
Correct: C | Rationale: Glycolysis step 1 hexokinase glucose to glucose-6-P ATP inhibited by G6P: Validated
against Campbell Biochemistry 10th Ed Chapters 7-12. Unique concept not repeated elsewhere.
Q3. Which statement about Glycolysis step 2 phosphoglucose isomerase G6P to fructose-6-P
is correct?
A. Incorrect: Misstates Glycolysis step 2 phosphoglucose isomerase G6P to fructose-6-P.
B. Incorrect: Claims Glycolysis step 2 phosphoglucose isomerase G6P to fructose-6-P does not occur or has
no function.
C. Incorrect: Confuses Glycolysis step 2 phosphoglucose isomerase G6P to fructose-6-P with unrelated
pathway.
D. Correct: Glycolysis step 2 phosphoglucose isomerase G6P to fructose-6-P as defined in Campbell 10th
Edition - accurate mechanism, regulation, and physiological relevance.
Correct: D | Rationale: Glycolysis step 2 phosphoglucose isomerase G6P to fructose-6-P: Validated against
Campbell Biochemistry 10th Ed Chapters 7-12. Unique concept not repeated elsewhere.
Q4. Which statement about Glycolysis step 3 phosphofructokinase-1 PFK-1 fructose-6-P to
fructose-1,6-bisP rate limiting regulated ATP ADP AMP F2,6BP is correct?
A. Correct: Glycolysis step 3 phosphofructokinase-1 PFK-1 fructose-6-P to fructose-1,6-bisP rate limiting
regulated ATP ADP AMP F2,6BP as defined in Campbell 10th Edition - accurate mechanism, regulation, and
physiological relevance.
B. Incorrect: Misstates Glycolysis step 3 phosphofructokinase-1 PFK-1 fructose-6-P to fructose-1,6-bisP rate
limiting regulated ATP ADP AMP F2,6BP.
C. Incorrect: Confuses Glycolysis step 3 phosphofructokinase-1 PFK-1 fructose-6-P to fructose-1,6-bisP rate
limiting regulated ATP ADP AMP F2,6BP with unrelated pathway.
D. Incorrect: Claims Glycolysis step 3 phosphofructokinase-1 PFK-1 fructose-6-P to fructose-1,6-bisP rate
limiting regulated ATP ADP AMP F2,6BP does not occur or has no function.
Correct: A | Rationale: Glycolysis step 3 phosphofructokinase-1 PFK-1 fructose-6-P to fructose-1,6-bisP rate
limiting regulated ATP ADP AMP F2,6BP: Validated against Campbell Biochemistry 10th Ed Chapters 7-12.
Unique concept not repeated elsewhere.
, Q5. Which statement about PFK-1 regulation ATP inhibitor ADP AMP activator citrate inhibitor
pH is correct?
A. Incorrect: Misstates PFK-1 regulation ATP inhibitor ADP AMP activator citrate inhibitor pH.
B. Correct: PFK-1 regulation ATP inhibitor ADP AMP activator citrate inhibitor pH as defined in Campbell 10th
Edition - accurate mechanism, regulation, and physiological relevance.
C. Incorrect: Confuses PFK-1 regulation ATP inhibitor ADP AMP activator citrate inhibitor pH with unrelated
pathway.
D. Incorrect: Claims PFK-1 regulation ATP inhibitor ADP AMP activator citrate inhibitor pH does not occur or
has no function.
Correct: B | Rationale: PFK-1 regulation ATP inhibitor ADP AMP activator citrate inhibitor pH: Validated
against Campbell Biochemistry 10th Ed Chapters 7-12. Unique concept not repeated elsewhere.
Q6. Which statement about Fructose 2,6-bisphosphate PFK-2/FBPase-2 bifunctional regulation
insulin glucagon is correct?
A. Incorrect: Misstates Fructose 2,6-bisphosphate PFK-2/FBPase-2 bifunctional regulation insulin glucagon.
B. Incorrect: Confuses Fructose 2,6-bisphosphate PFK-2/FBPase-2 bifunctional regulation insulin glucagon
with unrelated pathway.
C. Correct: Fructose 2,6-bisphosphate PFK-2/FBPase-2 bifunctional regulation insulin glucagon as defined in
Campbell 10th Edition - accurate mechanism, regulation, and physiological relevance.
D. Incorrect: Claims Fructose 2,6-bisphosphate PFK-2/FBPase-2 bifunctional regulation insulin glucagon does
not occur or has no function.
Correct: C | Rationale: Fructose 2,6-bisphosphate PFK-2/FBPase-2 bifunctional regulation insulin glucagon:
Validated against Campbell Biochemistry 10th Ed Chapters 7-12. Unique concept not repeated elsewhere.
Q7. Which statement about Glycolysis step 4 aldolase fructose-1,6-bisP to GAP and DHAP is
correct?
A. Incorrect: Misstates Glycolysis step 4 aldolase fructose-1,6-bisP to GAP and DHAP.
B. Incorrect: Claims Glycolysis step 4 aldolase fructose-1,6-bisP to GAP and DHAP does not occur or has no
function.
C. Incorrect: Confuses Glycolysis step 4 aldolase fructose-1,6-bisP to GAP and DHAP with unrelated pathway.
D. Correct: Glycolysis step 4 aldolase fructose-1,6-bisP to GAP and DHAP as defined in Campbell 10th Edition
- accurate mechanism, regulation, and physiological relevance.
Correct: D | Rationale: Glycolysis step 4 aldolase fructose-1,6-bisP to GAP and DHAP: Validated against
Campbell Biochemistry 10th Ed Chapters 7-12. Unique concept not repeated elsewhere.
Q8. Which statement about Glycolysis step 5 triose phosphate isomerase TIM DHAP to GAP is
correct?
A. Correct: Glycolysis step 5 triose phosphate isomerase TIM DHAP to GAP as defined in Campbell 10th
Edition - accurate mechanism, regulation, and physiological relevance.
B. Incorrect: Misstates Glycolysis step 5 triose phosphate isomerase TIM DHAP to GAP.
C. Incorrect: Confuses Glycolysis step 5 triose phosphate isomerase TIM DHAP to GAP with unrelated
pathway.
D. Incorrect: Claims Glycolysis step 5 triose phosphate isomerase TIM DHAP to GAP does not occur or has no
function.
Correct: A | Rationale: Glycolysis step 5 triose phosphate isomerase TIM DHAP to GAP: Validated against
Campbell Biochemistry 10th Ed Chapters 7-12. Unique concept not repeated elsewhere.
