UAMS MLT-MLS MICRO LAB FINAL
QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS ALREADY
PASSED!!!
Microbiology: Special Culture Media & Fastidious Pathogens Reference Matrix
The following matrix correctly pairs key fastidious or enteric pathogens with their proper
selective, differential, or enrichment media:
Pathogen / Organism Appropriate Special Media & Culture
Group Requirements
Blood Agar (SBA) / Mannitol Salt Agar (MSA)
Staphylococcus aureus (Note: MTM/NYC are used for Neisseria; MAC is for
Gram-negative enteric bacilli).
Thayer-Martin (MTM), New York City (NYC), or
Neisseria gonorrhoeae Martin-Lewis (Enriched chocolate-based selective
media containing antimicrobials).
MacConkey with Sorbitol (SMAC) (Ferments
Escherichia coli
sorbitol poorly, appearing colorless/clear compared to
O157:H7
normal sorbitol-fermenting pink E. coli).
Cysteine Blood Agar, Glucose-Cysteine Blood
Francisella tularensis Agar, or Buffered Charcoal-Yeast Extract (BCYE)
(Requires cysteine/cystine for growth).
Laked Blood Kanamycin-Vancomycin (LKV) Agar
Bacteroides or
or Bacteroides Bile Esculin (BBE) Agar (Selective
Prevotella
for anaerobic Gram-negative bacilli).
Cefsulodin-Irgasan-Novobiocin (CIN) Agar
Yersinia enterocolitica (Selective medium yielding "bull's-eye" colonies with
red centers).
Streptomyces / Enriched media with blood, serum, or ascitic fluid
Streptobacillus (e.g., Blood agar, serum broth; requires enriched
moniliformis media to support fastidious growth).
, PEA (Phenylethyl Alcohol) Blood Agar or Brucella
Anaerobic Gram-
Blood Agar (PEA inhibits facultative Gram-negative
Positive Cocci (GPC)
rods like E. coli while supporting anaerobes).
BACILLUS SPECIES
WHAT MO IS THIS ON BAP?
CHROMOBACTERIUM
What mo is this?
CHROMOBACTERIUM
NAME THE MO
PSEUDOMONAS
WHAT MO IS THIS ON BAP?
PSEUDOMONAS
WHAT MO IS THIS ON MHA
, FLAVOBACTERIIUM
WHAT MO IS THIS ON BAP
FLAVOBACTERIUM
WHAT MO IS THIS ON TSA
MICROCOCCUS
WHAT MO IS THIS
MICROCOCCUS
NAME MO ON BAP
E.COLI
NAME MO ON EMB
QUESTIONS AND ANSWERS WITH
COMPLETE SOLUTIONS ALREADY
PASSED!!!
Microbiology: Special Culture Media & Fastidious Pathogens Reference Matrix
The following matrix correctly pairs key fastidious or enteric pathogens with their proper
selective, differential, or enrichment media:
Pathogen / Organism Appropriate Special Media & Culture
Group Requirements
Blood Agar (SBA) / Mannitol Salt Agar (MSA)
Staphylococcus aureus (Note: MTM/NYC are used for Neisseria; MAC is for
Gram-negative enteric bacilli).
Thayer-Martin (MTM), New York City (NYC), or
Neisseria gonorrhoeae Martin-Lewis (Enriched chocolate-based selective
media containing antimicrobials).
MacConkey with Sorbitol (SMAC) (Ferments
Escherichia coli
sorbitol poorly, appearing colorless/clear compared to
O157:H7
normal sorbitol-fermenting pink E. coli).
Cysteine Blood Agar, Glucose-Cysteine Blood
Francisella tularensis Agar, or Buffered Charcoal-Yeast Extract (BCYE)
(Requires cysteine/cystine for growth).
Laked Blood Kanamycin-Vancomycin (LKV) Agar
Bacteroides or
or Bacteroides Bile Esculin (BBE) Agar (Selective
Prevotella
for anaerobic Gram-negative bacilli).
Cefsulodin-Irgasan-Novobiocin (CIN) Agar
Yersinia enterocolitica (Selective medium yielding "bull's-eye" colonies with
red centers).
Streptomyces / Enriched media with blood, serum, or ascitic fluid
Streptobacillus (e.g., Blood agar, serum broth; requires enriched
moniliformis media to support fastidious growth).
, PEA (Phenylethyl Alcohol) Blood Agar or Brucella
Anaerobic Gram-
Blood Agar (PEA inhibits facultative Gram-negative
Positive Cocci (GPC)
rods like E. coli while supporting anaerobes).
BACILLUS SPECIES
WHAT MO IS THIS ON BAP?
CHROMOBACTERIUM
What mo is this?
CHROMOBACTERIUM
NAME THE MO
PSEUDOMONAS
WHAT MO IS THIS ON BAP?
PSEUDOMONAS
WHAT MO IS THIS ON MHA
, FLAVOBACTERIIUM
WHAT MO IS THIS ON BAP
FLAVOBACTERIUM
WHAT MO IS THIS ON TSA
MICROCOCCUS
WHAT MO IS THIS
MICROCOCCUS
NAME MO ON BAP
E.COLI
NAME MO ON EMB
