Edexcel A Level Biology Paper 2 2026 Question Paper

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Centre Number Candidate Number




Pearson Edexcel Level 3 GCE
Friday 12 June 2026
Morning (Time: 1 hour 45 minutes) Paper
reference 9BI0/02
Biology B  


Advanced
PAPER 2: Advanced Physiology, Evolution and Ecology

You must have: Total Marks
Scientific calculator, HB pencil, ruler



Instructions
•• Use black ink or ball-point pen.
Fill in the boxes at the top of this page with your name,
centre number and candidate number.
• Answer all questions.
• Answer the questions in the spaces provided
– there may be more space than you need.
Information
• The total mark for this paper is 90.
• The marks for each question are shown in brackets
– use this as a guide as to how much time to spend on each question.
• Instructure
question(s) marked with an asterisk (*), marks will be awarded for your ability to
your answer logically showing how the points that you make are related or
follow on from each other where appropriate.
Advice
• Read each question carefully before you start to answer it.
• Checkanswer
Try to every question.
• your answers if you have time at the end.
Turn over


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, Answer ALL questions. Write your answers in the spaces provided.
Some questions must be answered with a cross in a box . If you change your mind about an




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answer, put a line through the box and then mark your new answer with a cross .
1 The diagram shows a molecule of tRNA (transfer RNA).

3’
A
C
C
5’ A
G C
C G
G C
A U
A U
U A
U A C U
U GACAC A
C G A A G
C CUCG CUGUG C
AG
G C
GAGCA UA
G G A G A C
C G G
C G
A U
G C
A U
C A
U U
G A A

(a) (i) Which row identifies bonds present in a molecule of tRNA?
(1)


Hydrogen bonds Phosphodiester bonds
A absent absent
B absent present
C present absent
D present present




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, (ii) The sequence of nucleotides in a section of messenger RNA (mRNA)
is AUGACCGGU.
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What is the sequence of nucleotides on the strand of DNA that was used as a
template for transcription of this section of mRNA?
(1)

A ATGTCCGGA

B AUGACCGGU

C TACTGGCCA

D UACUGGCCU

(iii) Which of the following statements about the genetic code are correct?
(1)
1. The code is non‑overlapping
2. Some amino acids have more than one codon
3. Some codons do not code for amino acids

A 1 and 2 only

B 1 and 3 only

C 1, 2 and 3

D 2 and 3 only

(b) Describe how translation at a ribosome produces a polypeptide.
(3)

................................ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .



................................ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .



................................ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .



................................ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .



................................ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .



................................ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .



................................ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .



................................ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .


(Total for Question 1 = 6 marks)




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, 2 The diagram shows part of the cell surface membrane.




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X




Y



(a) Name the parts labelled X and Y.
(1)

X ............................ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Y . ........................... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ........................................................................................................................................... .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

(b) Plant root hair cells take up nitrate ions. These ions are used to
synthesise molecules.
(i) Which row identifies biological molecules that contain nitrogen?
(1)

Cellulose DNA ligase enzyme Thymine

A absent present absent

B absent present present

C present absent present

D present present absent


(ii) Which one of the following describes the main method by which nitrate ions
can enter cells?
(1)

A active transport from a higher concentration to a lower
concentration using ATP
B active transport from a lower concentration to a higher
concentration using ATP
C facilitated diffusion from a higher concentration to a lower
concentration using ATP
D facilitated diffusion from a lower concentration to a higher
concentration using ATP




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