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what is the objective of aseptic technique and transferring culture -
ANSWER-to create a subculture of Staphylococcus Aureus
aseptic technique define and give some examples -ANSWER-any
procedure you do to help with contamination wipe table, flame the
loop, work close to Bunsen burner
whats a subculture? -ANSWER-transfer micro growth from one medium
to another
whats a pure culture? -ANSWER-culture of bacteria contaminating only
one strain
what medium do we work with in aseptic technique? -ANSWER-TSB-
trypticase soy broth
TSB is used over TSA why? -ANSWER-TSB is liquid and used to grow
aerobic bacteria
S. aureus is found where? -ANSWER-skin and respiratory tract
, S. aureus is a common cause of -ANSWER-food poison, skin infection,
respiratory disease, and staff
what is the objective of a 4 way streak -ANSWER-to obtain a pure
culture and dilute the bacteria
what is the bacteria used in 4 way streak? -ANSWER-micrococcus luteus
what are the characteristics of m. luteus? -ANSWER-obligate aerobic,
normal flora of the human skin, and isolated by flemming
how do you label the media? -ANSWER-initials, date, section, organism
name, procedure
why do you want to heat the loop until it is red hot? -ANSWER-you
want to kill any bacteria or microorganisms still on the loop
why do we let it cool? -ANSWER-if not, the loop would kill all the
bacteria in the tube
why do we flame the neck of the tube? -ANSWER-to keep
microorganisms away from the surface of the tube