CHEM 210 Module 4 | Biochemistry Exam Q&A | Portage
Learning
Here are 100 original practice questions for CHEM 210 Module 4 | Biochemistry. They
cover amino acid metabolism, protein turnover, the urea cycle, nitrogen disposal,
transamination, deamination, ammonia transport, amino acid disorders, and related
clinical applications.
Question 1:
The amino group of amino acids is removed primarily by:
A. Transamination
B. Oxidation
C. Reduction
D. Hydrolysis
Correct answer: A
Rationale: Transamination transfers the amino group to a keto acid, forming a new
amino acid.
Question 2:
Transamination reactions require which coenzyme?
A. Pyridoxal phosphate (B6)
B. Thiamine (B1)
C. Biotin
D. Vitamin C
Correct answer: A
Rationale: Pyridoxal phosphate (from vitamin B6) is the coenzyme for transamination.
Question 3:
The enzyme that catalyzes transamination is called:
A. Transaminase (aminotransferase)
B. Deaminase
C. Carboxylase
D. Hydrolase
Correct answer: A
Rationale: Transaminases (aminotransferases) catalyze transamination reactions.
,Question 4:
Which two aminotransferases are commonly measured in liver function tests?
A. ALT and AST
B. CK and LD
C. ALP and GGT
D. Amylase and lipase
Correct answer: A
Rationale: ALT (alanine aminotransferase) and AST (aspartate aminotransferase) are
liver enzymes.
Question 5:
Elevated ALT and AST suggest:
A. Liver damage
B. Kidney damage
C. Cardiac damage
D. Muscle damage only
Correct answer: A
Rationale: Elevated ALT and AST are markers of hepatocellular injury.
Question 6:
Oxidative deamination removes the amino group as:
A. Ammonia (NH₃)
B. Urea
C. Uric acid
D. Creatinine
Correct answer: A
Rationale: Oxidative deamination releases free ammonia.
Question 7:
Oxidative deamination occurs primarily in the:
A. Liver
B. Muscle
C. Brain
D. Kidney
Correct answer: A
Rationale: The liver is the primary site of oxidative deamination.
, Question 8:
The enzyme that catalyzes oxidative deamination of glutamate is:
A. Glutamate dehydrogenase
B. ALT
C. AST
D. Carbamoyl phosphate synthetase
Correct answer: A
Rationale: Glutamate dehydrogenase releases ammonia from glutamate.
Question 9:
Glutamate dehydrogenase uses which coenzyme?
A. NAD+ or NADP+
B. FAD
C. Biotin
D. TPP
Correct answer: A
Rationale: Glutamate dehydrogenase uses NAD+ or NADP+ as a coenzyme.
Question 10:
Ammonia is toxic to the brain primarily because it:
A. Depletes ATP and alters neurotransmitter balance
B. Increases ATP production
C. Has no effect on the brain
D. Increases oxygen delivery
Correct answer: A
Rationale: Ammonia depletes ATP and disrupts neurotransmission, causing
encephalopathy.
Question 11:
Ammonia is transported in the blood primarily as:
A. Glutamine
B. Urea
C. Uric acid
D. Creatinine
Learning
Here are 100 original practice questions for CHEM 210 Module 4 | Biochemistry. They
cover amino acid metabolism, protein turnover, the urea cycle, nitrogen disposal,
transamination, deamination, ammonia transport, amino acid disorders, and related
clinical applications.
Question 1:
The amino group of amino acids is removed primarily by:
A. Transamination
B. Oxidation
C. Reduction
D. Hydrolysis
Correct answer: A
Rationale: Transamination transfers the amino group to a keto acid, forming a new
amino acid.
Question 2:
Transamination reactions require which coenzyme?
A. Pyridoxal phosphate (B6)
B. Thiamine (B1)
C. Biotin
D. Vitamin C
Correct answer: A
Rationale: Pyridoxal phosphate (from vitamin B6) is the coenzyme for transamination.
Question 3:
The enzyme that catalyzes transamination is called:
A. Transaminase (aminotransferase)
B. Deaminase
C. Carboxylase
D. Hydrolase
Correct answer: A
Rationale: Transaminases (aminotransferases) catalyze transamination reactions.
,Question 4:
Which two aminotransferases are commonly measured in liver function tests?
A. ALT and AST
B. CK and LD
C. ALP and GGT
D. Amylase and lipase
Correct answer: A
Rationale: ALT (alanine aminotransferase) and AST (aspartate aminotransferase) are
liver enzymes.
Question 5:
Elevated ALT and AST suggest:
A. Liver damage
B. Kidney damage
C. Cardiac damage
D. Muscle damage only
Correct answer: A
Rationale: Elevated ALT and AST are markers of hepatocellular injury.
Question 6:
Oxidative deamination removes the amino group as:
A. Ammonia (NH₃)
B. Urea
C. Uric acid
D. Creatinine
Correct answer: A
Rationale: Oxidative deamination releases free ammonia.
Question 7:
Oxidative deamination occurs primarily in the:
A. Liver
B. Muscle
C. Brain
D. Kidney
Correct answer: A
Rationale: The liver is the primary site of oxidative deamination.
, Question 8:
The enzyme that catalyzes oxidative deamination of glutamate is:
A. Glutamate dehydrogenase
B. ALT
C. AST
D. Carbamoyl phosphate synthetase
Correct answer: A
Rationale: Glutamate dehydrogenase releases ammonia from glutamate.
Question 9:
Glutamate dehydrogenase uses which coenzyme?
A. NAD+ or NADP+
B. FAD
C. Biotin
D. TPP
Correct answer: A
Rationale: Glutamate dehydrogenase uses NAD+ or NADP+ as a coenzyme.
Question 10:
Ammonia is toxic to the brain primarily because it:
A. Depletes ATP and alters neurotransmitter balance
B. Increases ATP production
C. Has no effect on the brain
D. Increases oxygen delivery
Correct answer: A
Rationale: Ammonia depletes ATP and disrupts neurotransmission, causing
encephalopathy.
Question 11:
Ammonia is transported in the blood primarily as:
A. Glutamine
B. Urea
C. Uric acid
D. Creatinine
