Genetics Final Exam
With Correct
Questions And
Answers
For what is RNA-seq useful? - correct ans:to determine which genes are
active or inactive under typical conditions compared to experimental
conditions
What is the order of steps during RNA sequencing? - correct ans:1. mRNA
fragmentation
2. cDNA synthesis
3. Illumina sequencing
4. mapping to genes
Oligo dT primers are complementary to - correct ans:the 3' poly A tail of
mRNA
What is used to reverse transcribe fragmented mRNA into cDNA? - correct
ans:Random hexamers are used as primers
the sequence and expression patterns of all RNA transcripts in a particular
cell type or population of cells is known as - correct ans:Transcriptome
Which domains of GAL4 need to be in close proximity to form an active
transcription factor? - correct ans:GAL4 BD and GAL4 AD
,dDNAs are cloned next to the GAL4 AD forming ___ protein fusions that may
interact with the GAL4 BD ___ fusion proteins - correct ans:fish; bait
If two proteins do no interact, yeast cells will grow in medium lacking ___, but
not in medium lacking ___. - correct ans:tryptophan and leucine; tryptophan
leucine, and histidine
To what sequence are oligonucleotides on the flow cell complementary? -
correct ans:The adapter sequences of the DNA fragments
What does a DNA sequencing library contain? - correct ans:uniform sizes of
double stranded DNA fragments
How many cycles are used during Illumina sequencing by synthesis? - correct
ans:100 to 300 cycles
What is the correct order of steps in Next Generation WGS sequencing? -
correct ans:1. Cut many genome copies into random fragments
2. Sequence each fragment
3. Overlap sequence reads
4. Overlap contigs for complete sequence
After precipitation by centrifugation, where are the complexes of interest
found? - correct ans:In the pellet (the collection of cell at the bottom)
Regulatory proteins activate transcription of many genes simultaneously by -
correct ans:binding several promoter regions
The nucleosome contains - correct ans:DNA wrapped around histones
, What is the correct order of steps in ChiP? - correct ans:1. Cross-link proteins
to DNA
2. Break chromatin into small pieces
3. Add antibody to target protein and purify
4. Reverse cross-links to separate DNA and protein
Whole-genome shotgun sequencing is a method used to sequence genomic
DNA. Arrange the steps of whole-genome shotgun sequencing in order from
first to last - correct ans:1. Isolate genomic DNA
2. Break DNA into large fragments that are approximately 150,000 base pairs
in length
3. Insert DNA into BACs
4. Break DNA into fragments that are approximately 1000 base pairs in
length
5. Insert DNA into plasmids
6. Sequence DNA
7. Computationally assemble sequences from each BAC
8. Computationally assemble the genome from overlapping BACs
Place the steps of chromatin immuno-precipitation (ChIP) in order from first
to last.
Suppose you are studying a gene of interest. How would you be able to
determine if a histone with a particular modification binds to your gene of
interest? - correct ans:1. Crosslink DNA-binding proteins to DNA
2. Shear protein-bound DNA into fragments
3. Immunoprecipitate target protein
4. Reverse crosslinking of DNA and protein
5. Purify and sequence DNA
Use an antibody specific to the modified protein
With Correct
Questions And
Answers
For what is RNA-seq useful? - correct ans:to determine which genes are
active or inactive under typical conditions compared to experimental
conditions
What is the order of steps during RNA sequencing? - correct ans:1. mRNA
fragmentation
2. cDNA synthesis
3. Illumina sequencing
4. mapping to genes
Oligo dT primers are complementary to - correct ans:the 3' poly A tail of
mRNA
What is used to reverse transcribe fragmented mRNA into cDNA? - correct
ans:Random hexamers are used as primers
the sequence and expression patterns of all RNA transcripts in a particular
cell type or population of cells is known as - correct ans:Transcriptome
Which domains of GAL4 need to be in close proximity to form an active
transcription factor? - correct ans:GAL4 BD and GAL4 AD
,dDNAs are cloned next to the GAL4 AD forming ___ protein fusions that may
interact with the GAL4 BD ___ fusion proteins - correct ans:fish; bait
If two proteins do no interact, yeast cells will grow in medium lacking ___, but
not in medium lacking ___. - correct ans:tryptophan and leucine; tryptophan
leucine, and histidine
To what sequence are oligonucleotides on the flow cell complementary? -
correct ans:The adapter sequences of the DNA fragments
What does a DNA sequencing library contain? - correct ans:uniform sizes of
double stranded DNA fragments
How many cycles are used during Illumina sequencing by synthesis? - correct
ans:100 to 300 cycles
What is the correct order of steps in Next Generation WGS sequencing? -
correct ans:1. Cut many genome copies into random fragments
2. Sequence each fragment
3. Overlap sequence reads
4. Overlap contigs for complete sequence
After precipitation by centrifugation, where are the complexes of interest
found? - correct ans:In the pellet (the collection of cell at the bottom)
Regulatory proteins activate transcription of many genes simultaneously by -
correct ans:binding several promoter regions
The nucleosome contains - correct ans:DNA wrapped around histones
, What is the correct order of steps in ChiP? - correct ans:1. Cross-link proteins
to DNA
2. Break chromatin into small pieces
3. Add antibody to target protein and purify
4. Reverse cross-links to separate DNA and protein
Whole-genome shotgun sequencing is a method used to sequence genomic
DNA. Arrange the steps of whole-genome shotgun sequencing in order from
first to last - correct ans:1. Isolate genomic DNA
2. Break DNA into large fragments that are approximately 150,000 base pairs
in length
3. Insert DNA into BACs
4. Break DNA into fragments that are approximately 1000 base pairs in
length
5. Insert DNA into plasmids
6. Sequence DNA
7. Computationally assemble sequences from each BAC
8. Computationally assemble the genome from overlapping BACs
Place the steps of chromatin immuno-precipitation (ChIP) in order from first
to last.
Suppose you are studying a gene of interest. How would you be able to
determine if a histone with a particular modification binds to your gene of
interest? - correct ans:1. Crosslink DNA-binding proteins to DNA
2. Shear protein-bound DNA into fragments
3. Immunoprecipitate target protein
4. Reverse crosslinking of DNA and protein
5. Purify and sequence DNA
Use an antibody specific to the modified protein
