MLS IMMUNOHEMATOLOGY FINAL
CLINICAL REVIEW EXAM
Which blood component contains the most factor VIII concentrate relative to the unit
volume? - Correct Answers -Cryoprecipitated AHF
A sample has reactions occurring at immediate spin and AHG in a panel that show
varying strengths. There is no obvious pattern that matches a particular panel cell or
single antigen profile and the auto-control was negative. Which of the following is most
likely the cause? - Correct Answers -An IgM and an IgG antibody
A rosette test to screen for fetal maternal hemorrhage should not be performed if the
newborn has a weak-D antigen. - Correct Answers -True: false negatives could occur
What should be done if all forward and reverse ABO results are negative? - Correct
Answers -Incubate at room temperature of 4˚ C to enhance weak expression.
If a pregnant female who was injected with RhIg antenatally has anti-D at delivery,
routine antibody titration to determine the titer of the anti-D is considered to be a good
practice. - Correct Answers -False
A pregnant female has been injected with RhIg antenatally and has a positive antibody
screen at delivery. If the antibody has been confirmed as anti-D alone and reacts only
weakly (1+ in the IAT), the anti-D is definitely passive. - Correct Answers -False. Only if
the anti-D was strong could passive be ruled out
What is the most prudent step to follow to select units for cross-match after recipient
antibodies have been identified? - Correct Answers -Antigen type the patient cells and
any donor cells to be cross-matched.
A group Oh Bombay individual's blood specimen can be differentiated from blood
specimens of normal group O person's by: - Correct Answers -Anti-H in serum
RhIg prophylaxis is typically given antenatally to Rh negative pregnant females without
knowing the Rh type of the baby. - Correct Answers -True
What type of packed red blood cells can be transfused to a group O patient? - Correct
Answers -Group O only
RhIg prevents anti-D production mainly by clearing antibody sensitized D-positive RBC's
from maternal circulation - Correct Answers -False
, Phenotypes of offspring of type O parent and type A parent - Correct Answers -O and A
only
Which class of immunoglobulin is implicated in hemolytic disease of the fetus and
newborn? - Correct Answers -IgG
Which is the first marker (antigen or antibody) which will become positive after exposure
to hepatitis B? - Correct Answers -HBsAg
What action should be taken if a large clot is noticed in a red cell unit while the product
is being prepared for release to the patient? - Correct Answers -Do not issue the unit
At 28 weeks gestation an Rh-negative mother had a negative antibody screen and was
injected with RhIg. Anti-D is detected in the mother's serum at the time of delivery. A
panel is performed that excludes the presence of other antibodies. The most likely
conclusion would be passive anti-D due to RhIg administration. - Correct Answers -True
Reverse typing is done using known antisera to detect ABO antigens present on the
patient red cells - Correct Answers -False-
Reverse typing is done to detect patient antibodies in serum.
The most recent algorithm for HIV confirmatory testing in patients who are positive by
HIV 1/2 antigen/antibody combination immunoassay is the - Correct Answers -
HIV1/HIV2 antibody differentiation immunoassay
The cause of the most severe life threatening hemolytic transfusion reaction is - Correct
Answers -Anti-A, Anti-B, and Anti-AB
A patient who received an autologous donation three weeks ago, can be reliably antigen
typed - Correct Answers -True. It is an autologous donation not allogeneic.
The most definite indication that a patient has been sensitized to a specific red cell
antigen is - Correct Answers -A positive post transfusion DAT in a patient with a
previously negative DAT
A urticarial transfusion reaction is characterized by - Correct Answers -Rash and Hives
Which test best correlates with the severity of hemolytic disease of a newborn? -
Correct Answers -Amniotic fluid bilirubin
FFP that has been thawed at 30-37˚C and maintained at 1-6˚C must be transfused
within ______ hours after it has been thawed - Correct Answers -24
IgG coated red cells are added to negative antiglobulin test to detect which source of
error? - Correct Answers -Failure to add Coombs serum
CLINICAL REVIEW EXAM
Which blood component contains the most factor VIII concentrate relative to the unit
volume? - Correct Answers -Cryoprecipitated AHF
A sample has reactions occurring at immediate spin and AHG in a panel that show
varying strengths. There is no obvious pattern that matches a particular panel cell or
single antigen profile and the auto-control was negative. Which of the following is most
likely the cause? - Correct Answers -An IgM and an IgG antibody
A rosette test to screen for fetal maternal hemorrhage should not be performed if the
newborn has a weak-D antigen. - Correct Answers -True: false negatives could occur
What should be done if all forward and reverse ABO results are negative? - Correct
Answers -Incubate at room temperature of 4˚ C to enhance weak expression.
If a pregnant female who was injected with RhIg antenatally has anti-D at delivery,
routine antibody titration to determine the titer of the anti-D is considered to be a good
practice. - Correct Answers -False
A pregnant female has been injected with RhIg antenatally and has a positive antibody
screen at delivery. If the antibody has been confirmed as anti-D alone and reacts only
weakly (1+ in the IAT), the anti-D is definitely passive. - Correct Answers -False. Only if
the anti-D was strong could passive be ruled out
What is the most prudent step to follow to select units for cross-match after recipient
antibodies have been identified? - Correct Answers -Antigen type the patient cells and
any donor cells to be cross-matched.
A group Oh Bombay individual's blood specimen can be differentiated from blood
specimens of normal group O person's by: - Correct Answers -Anti-H in serum
RhIg prophylaxis is typically given antenatally to Rh negative pregnant females without
knowing the Rh type of the baby. - Correct Answers -True
What type of packed red blood cells can be transfused to a group O patient? - Correct
Answers -Group O only
RhIg prevents anti-D production mainly by clearing antibody sensitized D-positive RBC's
from maternal circulation - Correct Answers -False
, Phenotypes of offspring of type O parent and type A parent - Correct Answers -O and A
only
Which class of immunoglobulin is implicated in hemolytic disease of the fetus and
newborn? - Correct Answers -IgG
Which is the first marker (antigen or antibody) which will become positive after exposure
to hepatitis B? - Correct Answers -HBsAg
What action should be taken if a large clot is noticed in a red cell unit while the product
is being prepared for release to the patient? - Correct Answers -Do not issue the unit
At 28 weeks gestation an Rh-negative mother had a negative antibody screen and was
injected with RhIg. Anti-D is detected in the mother's serum at the time of delivery. A
panel is performed that excludes the presence of other antibodies. The most likely
conclusion would be passive anti-D due to RhIg administration. - Correct Answers -True
Reverse typing is done using known antisera to detect ABO antigens present on the
patient red cells - Correct Answers -False-
Reverse typing is done to detect patient antibodies in serum.
The most recent algorithm for HIV confirmatory testing in patients who are positive by
HIV 1/2 antigen/antibody combination immunoassay is the - Correct Answers -
HIV1/HIV2 antibody differentiation immunoassay
The cause of the most severe life threatening hemolytic transfusion reaction is - Correct
Answers -Anti-A, Anti-B, and Anti-AB
A patient who received an autologous donation three weeks ago, can be reliably antigen
typed - Correct Answers -True. It is an autologous donation not allogeneic.
The most definite indication that a patient has been sensitized to a specific red cell
antigen is - Correct Answers -A positive post transfusion DAT in a patient with a
previously negative DAT
A urticarial transfusion reaction is characterized by - Correct Answers -Rash and Hives
Which test best correlates with the severity of hemolytic disease of a newborn? -
Correct Answers -Amniotic fluid bilirubin
FFP that has been thawed at 30-37˚C and maintained at 1-6˚C must be transfused
within ______ hours after it has been thawed - Correct Answers -24
IgG coated red cells are added to negative antiglobulin test to detect which source of
error? - Correct Answers -Failure to add Coombs serum
