IMMUNOHEMATOLOGY TEST 4—
ELUTION QUESTIONS AND ANSWERS
What is an elution? - Correct Answers -The removal of antibodies from the RBC surface
by altering the attractive forces between antigens and antibodies.
What is an eluate? - Correct Answers -A fluid medium containing the antibodies that
have been deliberately removed from RBCs, allowing for antibody identification.
What are the 2 main instances when you would perform an elution? - Correct Answers -
1. When the DAT is positive with anti-IgG AHG reagent.
2. To prepare antibody-free RBCs for use in phenotyping or autologous adsorption
studies.
What is the purpose of a total elution? - Correct Answers -A total elution removes the
antibody coating the RBCs and destroys the antigens to which they were attached. This
allows for identification of the antibody that had been coating the cells.
What is the purpose of a partial elution? - Correct Answers -Partial elution removes the
antibody but allows the antigen to remain intact. The treated cells may then be antigen
typed or used in adsorption procedures.
When trying to free antibodies from cells with a positive DAT, is a total or partial elution
usually required? - Correct Answers -Total (and the eluate is collected and tested)
When trying to prepare antibody-free RBCs for use in phenotyping or autologous
adsorption studies, is a total or partial elution usually required? - Correct Answers -
Partial (and the eluate is not collected or tested)
What is the application of a total elution? - Correct Answers -To determine which
antibodies are responsible for the positive DAT leading to HDFN, HTR, and/or acquired
hemolytic anemia (immune or drug induced).
Will RBCs with a positive DAT due to complement have reactivity in the eluate? -
Correct Answers -No
What are the 4 main types of elution? - Correct Answers -1. Temperature (56 C, 45 C,
and Lui freeze)
2. pH (gamma Elu-Kit II, EGA-EDTA Glycine-Acid)
3. Chemical elution (ZZAP, Chloroquine, 2-Mercaptoethanol)
ELUTION QUESTIONS AND ANSWERS
What is an elution? - Correct Answers -The removal of antibodies from the RBC surface
by altering the attractive forces between antigens and antibodies.
What is an eluate? - Correct Answers -A fluid medium containing the antibodies that
have been deliberately removed from RBCs, allowing for antibody identification.
What are the 2 main instances when you would perform an elution? - Correct Answers -
1. When the DAT is positive with anti-IgG AHG reagent.
2. To prepare antibody-free RBCs for use in phenotyping or autologous adsorption
studies.
What is the purpose of a total elution? - Correct Answers -A total elution removes the
antibody coating the RBCs and destroys the antigens to which they were attached. This
allows for identification of the antibody that had been coating the cells.
What is the purpose of a partial elution? - Correct Answers -Partial elution removes the
antibody but allows the antigen to remain intact. The treated cells may then be antigen
typed or used in adsorption procedures.
When trying to free antibodies from cells with a positive DAT, is a total or partial elution
usually required? - Correct Answers -Total (and the eluate is collected and tested)
When trying to prepare antibody-free RBCs for use in phenotyping or autologous
adsorption studies, is a total or partial elution usually required? - Correct Answers -
Partial (and the eluate is not collected or tested)
What is the application of a total elution? - Correct Answers -To determine which
antibodies are responsible for the positive DAT leading to HDFN, HTR, and/or acquired
hemolytic anemia (immune or drug induced).
Will RBCs with a positive DAT due to complement have reactivity in the eluate? -
Correct Answers -No
What are the 4 main types of elution? - Correct Answers -1. Temperature (56 C, 45 C,
and Lui freeze)
2. pH (gamma Elu-Kit II, EGA-EDTA Glycine-Acid)
3. Chemical elution (ZZAP, Chloroquine, 2-Mercaptoethanol)
