Actual Bio 1108 Exam II | UPDATED Questions with 100%
Verified Answers
Q1: Traditional Breeding vs.
A: precise genes within 1 generation
Q2: Transgenic Organisims
A: biotech creation; receives DNA from different speceis
Q3: Restriction Enzymes
A: cut DNA at specific sequence on opposite ends of restriction site
Q4: Sticky Ends
A: cut DNA with loose ends that can bind to plasmid or DNA of another species
Q5: Antibiotic Resistance
A: genes into DNA by plasmids
Q6: Rice and Bean Expo.
A: LacZ gene override by amipicilin for higher iron concentraiton from beans to rice
Q7: LacZ produces enzyme turning bacteria
A: blue
Q8: LacZ produces enzyme turning absence of bacteria
A: white
Q9: How many steps in Bean and Rice LacZ process
A: 5 to 6
, Q10: Steps of Bean and Rice LacZ expo.
A: 1. cut plasmids and bean DNA with same restriction enzyme in the middle of LacZ gene.
Plasmids will anneal at sticky ends
2. Restricition Enzyme cleans complementary sticky ends on foreign DNA fragments and
plasmid; DNA is inserted into plasmids when sticky ends anneal
3. added DNA ligase to reattach DNA forming recombinant plasmids
4. plasmids with growing bactera: no plasmids, non recombinant plasmids, and desirced
recombinant plasmids
5. Bacteria grow with amipicilin and 1. no plasmids killled with ampicillin 2.
nonrecombinant plasmids turn blue and removed 3. recombinant plasmids turn white
Q11: cDNA
A: genetic complementary of DNA
Q12: Bacteria cannot process ____ to make recombinant insulin
A: introns
Q13: cDNA to DNA for insulin steps
A: 1. take mRNA produce from human insulin gene
2. add reverse transcripatase to make cDNA from mRNA
3. DNA from mRNA into bacteria
4. PCR to clone copies
Q14: Bacteria from ____ withstand ___
A: hot sulfer springs; PCR
Q15: PCR
A: Polymerase Chain Reaction
Q16: PCR Steps
A: 1. Denature breaks DNA hydrogen bonds (hot)
2. Annealing cools down and adds primers to attach DNA
3. Elongation warms again for DNA polymerase to elongate strands
Q17: Gene Therapy
A: started in 1980s, 5 parts
Verified Answers
Q1: Traditional Breeding vs.
A: precise genes within 1 generation
Q2: Transgenic Organisims
A: biotech creation; receives DNA from different speceis
Q3: Restriction Enzymes
A: cut DNA at specific sequence on opposite ends of restriction site
Q4: Sticky Ends
A: cut DNA with loose ends that can bind to plasmid or DNA of another species
Q5: Antibiotic Resistance
A: genes into DNA by plasmids
Q6: Rice and Bean Expo.
A: LacZ gene override by amipicilin for higher iron concentraiton from beans to rice
Q7: LacZ produces enzyme turning bacteria
A: blue
Q8: LacZ produces enzyme turning absence of bacteria
A: white
Q9: How many steps in Bean and Rice LacZ process
A: 5 to 6
, Q10: Steps of Bean and Rice LacZ expo.
A: 1. cut plasmids and bean DNA with same restriction enzyme in the middle of LacZ gene.
Plasmids will anneal at sticky ends
2. Restricition Enzyme cleans complementary sticky ends on foreign DNA fragments and
plasmid; DNA is inserted into plasmids when sticky ends anneal
3. added DNA ligase to reattach DNA forming recombinant plasmids
4. plasmids with growing bactera: no plasmids, non recombinant plasmids, and desirced
recombinant plasmids
5. Bacteria grow with amipicilin and 1. no plasmids killled with ampicillin 2.
nonrecombinant plasmids turn blue and removed 3. recombinant plasmids turn white
Q11: cDNA
A: genetic complementary of DNA
Q12: Bacteria cannot process ____ to make recombinant insulin
A: introns
Q13: cDNA to DNA for insulin steps
A: 1. take mRNA produce from human insulin gene
2. add reverse transcripatase to make cDNA from mRNA
3. DNA from mRNA into bacteria
4. PCR to clone copies
Q14: Bacteria from ____ withstand ___
A: hot sulfer springs; PCR
Q15: PCR
A: Polymerase Chain Reaction
Q16: PCR Steps
A: 1. Denature breaks DNA hydrogen bonds (hot)
2. Annealing cools down and adds primers to attach DNA
3. Elongation warms again for DNA polymerase to elongate strands
Q17: Gene Therapy
A: started in 1980s, 5 parts
