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,Anatomy
Study of body parts/structures/organs and how they are constructed. Ex: Glands
Also known as how things come together
Physiology
The study of how body parts, structures, and organs function.
Gross Anatomy and Microscopic Anatomy
What are the two specific types of anatomy?
Gross Anatomy
Things you can see with the naked eye or handheld magnifying glass like structures and
organs
Microscopic Anatomy
Must use some type of microscope to view cells, tissues, and organs
Light Microscope and Electron Miscroscope
What are the two specific types of microscopes?
,Structure determines function
What is the universal anatomy and physiology statement?
Magnification and Resolution
What two things do microscopes do?
Magnification
The process of enlarging an object
Resolution
The ability of a microscope to distinguish between two objects clearly and distinctly
Light Microscope (LM)
Used to see cells by using light to carry the image to the eye
Can only magnify up to 10,000x
Uses halogen or LED bulbs
Uses water based stains to provide contrast
Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM)
What are the two specific types of electron microscopes?
, Transmission Electron Microscope (TEM)
Shows ultra fine detail and allows visualization of internal structures of cells, chemical
elements, and organelles by sending electricity through the sample
Magnifies up to 500,000x
Produces the images on a computer screen
Scanning Electron Microscope (SEM)
Allows visualization of surface structures of objects even nonliving chemical elements
Magnifies up to 250,000x
Micrograph
What is the pictures that is taken by electron microscopes called?
Histotechnique
The process of making slides is called what?
1) Obtain a dead or alive sample
2)Kill the sample with preservatives
3) Cut the sample into smaller pieces
4) Dehydrate the sample more by putting it in different concentrations of alcohol
5) Clear the sample of any remaining alcohol by using toluene
6) Embed the sample into wax and bake for two weeks
7) Section (cut) the sample using a microtone to make thin slices
8) Mount the thin section on slide using a hotplate to adhere it to the slide
9)Add stain to slide for contrast
10) Add coverslip and observe under microscope
What is the process of making a LM slide? (Histotechnique)
Formaldehyde, freezing the sample, alcohol, and gas
What are the types of preservatives used to kill sample?