PPATH 211 Plant Pathology Practical Experiment 1 With Original Lab Photos | Microscopy Sterilization Culture Media PDA Preparation | 8 Pages Handwritten Notes | BSc Agriculture 6th Dean

PPATH 211 PLANT PATHOLOGY PRACTICAL EXPERIMENT 1 - COMPLETE 8 PAGES HANDWRITTEN NOTES WITH ORIGINAL LAB PHOTOS | BSc Agriculture 3rd Semester 6th Dean This is the complete handwritten practical record for PPATH 211 / PPATH 311 Plant Pathology Practical Experiment 1, with original lab photos clicked during practical class. This is the most important first experiment asked in practical record and external exam. WHAT IS INCLUDED IN THIS 8 PAGES: 1. STUDY OF COMPOUND MICROSCOPE: - Detailed diagram of compound microscope with labelling - Eyepiece, Objective lens (10x, 40x, 100x), Stage, Condenser, Iris diaphragm, Coarse and Fine adjustment. - Functions of each part explained in handwritten notes. - Handling and care of microscope. - Original lab photo of compound microscope from lab. 2. STERILIZATION - PRINCIPLE AND METHODS: - Definition of sterilization and its importance in plant pathology. - Different methods: a) Dry heat - Hot air oven (160-180°C for 2 hours) - for glassware like petriplates, test tubes. b) Moist heat - Autoclave (121°C, 15 psi for 15-20 min) - for media, water. c) Chemical - Alcohol, formaldehyde. d) Filtration. - Original photos of autoclave and hot air oven from practical lab. - Neat handwritten procedure how to operate. 3. CULTURE MEDIA PREPARATION: - What is culture media, types - Natural, Semi-synthetic, Synthetic. - PDA (Potato Dextrose Agar) - Most widely used for fungi - Composition: 200g potato, 20g dextrose, 20g agar, 1000ml water. - Nutrient Agar for bacteria. - Step by step handwritten procedure for PDA preparation - Boiling, straining, adding agar, autoclaving, pouring in petriplates. - Precautions. - Original photos of PDA plates and pouring. WHY THIS NOTE IS SPECIAL: - Includes ORIGINAL LAB PHOTOS - No other note on Docmerit/Stuvia has real lab photos, only diagrams. This helps you draw exactly for record and viva. - Handwritten, neat and clean, directly usable for practical record writing. - Includes both diagrams and photos with captions - Fig 1, Fig 2 as required by professor. - As per ANGRAU, PJTSAU, all SAUs under ICAR 6th Dean syllabus for PPATH 211, PPATH 311. - Covers viva questions - What is sterilization, why autoclave is used, composition of PDA. Useful for: BSc Agriculture 2nd year / 3rd semester, BSc Horticulture, BSc Forestry, Diploma in Agriculture students. Also useful for practical record completion, external practical exam and ICAR JRF preparation. Total Pages: 8 (Handwritten + 3-4 original lab photos included inside PDF) Format: PDF University: ANGRAU (Applicable to all ICAR universities) Experiment No: 1 Tags: plant pathology practical, PPATH 211, microscopy, sterilization, autoclave, hot air oven, culture media, PDA preparation, potato dextrose agar, BSc agriculture practical notes, ANGRAU, original lab photos