CHEM 210 BIOCHEMISTRY MODULE 5
& FINAL EXAM COMPREHENSIVE
QUESTIONS AND ANSWERS 2026/2027
UPDATE || 100% GUARANTEED PASS
1. Which enzyme is responsible for the primary regulatory step in glycolysis, catalyzing the
conversion of Fructose-6-Phosphate to Fructose-1,6-Bisphosphate?
A. Hexokinase
B. Pyruvate Kinase
C. Phosphofructokinase-1
D. Phosphoglycerate Kinase
Answer: C
Conceptual Explanation: Phosphofructokinase-1 (PFK-1) is the rate-limiting enzyme of
glycolysis and is highly regulated by ATP and Fructose-2,6-bisphosphate.
2. In the Malate-Aspartate Shuttle, which molecule is transported into the mitochondrial
matrix to carry reducing equivalents?
A. Oxaloacetate
B. NADH
C. Aspartate
,D. Malate
Answer: D
Conceptual Explanation: NADH cannot cross the inner mitochondrial membrane, so its
electrons are transferred to oxaloacetate to form malate, which is then transported into the
matrix.
3. Which of the following enzymes is unique to gluconeogenesis and bypasses the irreversible
step catalyzed by Pyruvate Kinase?
A. Glucose-6-Phosphatase
B. Fructose-1,6-Bisphosphatase
C. Pyruvate Carboxylase
D. Enolase
Answer: C
Conceptual Explanation: Pyruvate Carboxylase (along with PEP carboxykinase) is
required to convert pyruvate back to phosphoenolpyruvate, bypassing the irreversible
pyruvate kinase reaction.
4. The oxidation of one mole of glucose via aerobic glycolysis, the PDH complex, and the TCA
cycle produces how many moles of CO2?
A. 6
B. 4
, C. 2
D. 12
Answer: A
Conceptual Explanation: Each glucose has 6 carbons. Two are lost at the PDH step and
four are lost in the TCA cycle (two per acetyl-CoA).
5. Which complex of the Electron Transport Chain does not pump protons across the inner
mitochondrial membrane?
A. Complex I
B. Complex IV
C. Complex III
D. Complex II
Answer: D
Conceptual Explanation: Complex II (Succinate Dehydrogenase) transfers electrons from
FADH2 to Coenzyme Q but lacks the energy to pump protons.
6. A deficiency in which enzyme results in Von Gierke’s Disease (Glycogen Storage Disease
Type I)?
A. Glycogen Phosphorylase
B. Debranching Enzyme
C. Acid Alpha-Glucosidase
& FINAL EXAM COMPREHENSIVE
QUESTIONS AND ANSWERS 2026/2027
UPDATE || 100% GUARANTEED PASS
1. Which enzyme is responsible for the primary regulatory step in glycolysis, catalyzing the
conversion of Fructose-6-Phosphate to Fructose-1,6-Bisphosphate?
A. Hexokinase
B. Pyruvate Kinase
C. Phosphofructokinase-1
D. Phosphoglycerate Kinase
Answer: C
Conceptual Explanation: Phosphofructokinase-1 (PFK-1) is the rate-limiting enzyme of
glycolysis and is highly regulated by ATP and Fructose-2,6-bisphosphate.
2. In the Malate-Aspartate Shuttle, which molecule is transported into the mitochondrial
matrix to carry reducing equivalents?
A. Oxaloacetate
B. NADH
C. Aspartate
,D. Malate
Answer: D
Conceptual Explanation: NADH cannot cross the inner mitochondrial membrane, so its
electrons are transferred to oxaloacetate to form malate, which is then transported into the
matrix.
3. Which of the following enzymes is unique to gluconeogenesis and bypasses the irreversible
step catalyzed by Pyruvate Kinase?
A. Glucose-6-Phosphatase
B. Fructose-1,6-Bisphosphatase
C. Pyruvate Carboxylase
D. Enolase
Answer: C
Conceptual Explanation: Pyruvate Carboxylase (along with PEP carboxykinase) is
required to convert pyruvate back to phosphoenolpyruvate, bypassing the irreversible
pyruvate kinase reaction.
4. The oxidation of one mole of glucose via aerobic glycolysis, the PDH complex, and the TCA
cycle produces how many moles of CO2?
A. 6
B. 4
, C. 2
D. 12
Answer: A
Conceptual Explanation: Each glucose has 6 carbons. Two are lost at the PDH step and
four are lost in the TCA cycle (two per acetyl-CoA).
5. Which complex of the Electron Transport Chain does not pump protons across the inner
mitochondrial membrane?
A. Complex I
B. Complex IV
C. Complex III
D. Complex II
Answer: D
Conceptual Explanation: Complex II (Succinate Dehydrogenase) transfers electrons from
FADH2 to Coenzyme Q but lacks the energy to pump protons.
6. A deficiency in which enzyme results in Von Gierke’s Disease (Glycogen Storage Disease
Type I)?
A. Glycogen Phosphorylase
B. Debranching Enzyme
C. Acid Alpha-Glucosidase
