CHEM 210 BIOCHEMISTRY
COMPREHENSIVE MODULE 3 AND
FINAL EXAM QUESTIONS AND
ANSWERS 2026/2027 UPDATE || 100%
GUARANTEED PASS
1. Which of the following amino acids is most likely to be found in the interior of a globular
protein?
A. Aspartate
B. Lysine
C. Isoleucine
D. Serine
Answer: C
Conceptual Explanation: Isoleucine is nonpolar and hydrophobic, causing it to be
sequestered in the protein core away from water.
2. In the Michaelis-Menten model, what does the constant Km represent?
A. The maximum velocity of the reaction
B. The substrate concentration at half Vmax
C. The rate of enzyme-substrate complex dissociation
,D. The total enzyme concentration
Answer: B
Conceptual Explanation: Km is defined as the substrate concentration at which the
reaction rate is half of its maximal value.
3. A competitive inhibitor has what effect on the Lineweaver-Burk plot?
A. Changes the slope but not the y-intercept
B. Decreases the x-intercept value
C. Increases the y-intercept
D. Leaves the slope unchanged
Answer: A
Conceptual Explanation: Competitive inhibitors increase Km (moving x-intercept right)
but do not change Vmax (y-intercept remains constant).
4. Which bond stabilizes the alpha-helix secondary structure of proteins?
A. Disulfide bridges
B. Ionic interactions between R-groups
C. Hydrogen bonds between peptide backbone atoms
D. Hydrophobic interactions
Answer: C
, Conceptual Explanation: Alpha-helices are stabilized by hydrogen bonding between the
carbonyl oxygen of one amino acid and the amide hydrogen four residues away.
5. What is the primary function of a catalyst in a biochemical reaction?
A. To lower the activation energy
B. To increase the Gibbs free energy change
C. To shift the equilibrium toward the products
D. To decrease the entropy of the system
Answer: A
Conceptual Explanation: Catalysts/enzymes lower the activation energy barrier, allowing
the reaction to proceed faster without changing the equilibrium.
6. Which molecule acts as the primary CO2 carrier in the blood?
A. Carbaminohemoglobin
B. Bicarbonate ion
C. Dissolved CO2 gas
D. Carbonic acid
Answer: B
Conceptual Explanation: Approximately 70% of CO2 is transported in the blood as
bicarbonate ions produced via carbonic anhydrase.
COMPREHENSIVE MODULE 3 AND
FINAL EXAM QUESTIONS AND
ANSWERS 2026/2027 UPDATE || 100%
GUARANTEED PASS
1. Which of the following amino acids is most likely to be found in the interior of a globular
protein?
A. Aspartate
B. Lysine
C. Isoleucine
D. Serine
Answer: C
Conceptual Explanation: Isoleucine is nonpolar and hydrophobic, causing it to be
sequestered in the protein core away from water.
2. In the Michaelis-Menten model, what does the constant Km represent?
A. The maximum velocity of the reaction
B. The substrate concentration at half Vmax
C. The rate of enzyme-substrate complex dissociation
,D. The total enzyme concentration
Answer: B
Conceptual Explanation: Km is defined as the substrate concentration at which the
reaction rate is half of its maximal value.
3. A competitive inhibitor has what effect on the Lineweaver-Burk plot?
A. Changes the slope but not the y-intercept
B. Decreases the x-intercept value
C. Increases the y-intercept
D. Leaves the slope unchanged
Answer: A
Conceptual Explanation: Competitive inhibitors increase Km (moving x-intercept right)
but do not change Vmax (y-intercept remains constant).
4. Which bond stabilizes the alpha-helix secondary structure of proteins?
A. Disulfide bridges
B. Ionic interactions between R-groups
C. Hydrogen bonds between peptide backbone atoms
D. Hydrophobic interactions
Answer: C
, Conceptual Explanation: Alpha-helices are stabilized by hydrogen bonding between the
carbonyl oxygen of one amino acid and the amide hydrogen four residues away.
5. What is the primary function of a catalyst in a biochemical reaction?
A. To lower the activation energy
B. To increase the Gibbs free energy change
C. To shift the equilibrium toward the products
D. To decrease the entropy of the system
Answer: A
Conceptual Explanation: Catalysts/enzymes lower the activation energy barrier, allowing
the reaction to proceed faster without changing the equilibrium.
6. Which molecule acts as the primary CO2 carrier in the blood?
A. Carbaminohemoglobin
B. Bicarbonate ion
C. Dissolved CO2 gas
D. Carbonic acid
Answer: B
Conceptual Explanation: Approximately 70% of CO2 is transported in the blood as
bicarbonate ions produced via carbonic anhydrase.
